Product Description
Getting V4 libraries onto the sequencer faster frees time for analysis. This Zymo Research kit uses a premixed amplification plate and a single PCR step to bring hands-on library preparation down to about 30 minutes, making high-throughput 16S V4 sequencing practical for busy microbiome labs.
This listing is for catalog number D6430-PS4: Quick-16S Plus NGS Library Prep Kit (V4) (96 preps) with Index Primer Set 4. DNA is added to a premixed plate where one qPCR/PCR reaction both amplifies the V4 region of the 16S rRNA gene and attaches barcodes. Libraries are pooled by equal volume and the pool is cleaned up once, replacing many individual bead purifications, with no TapeStation or gel quantification required. Each kit provides 96 preps, with four index primer sets available. LIGHT LABS stocks the Zymo Research line, so kits, buffers and refills are always within reach.
Key Features
- Premixed amplification plate – Ready-to-use plate simplifies setup.
- Single-PCR workflow – Combines V4 amplification and barcode addition in one qPCR/PCR step.
- Pool by equal volume – No per-sample normalization before pooling.
- One final clean-up – A single pooled clean-up replaces many bead purifications.
- About 30 minutes hands-on – No separate TapeStation or gel quantification needed.
- Automation-friendly – Designed for liquid-handling platforms.
Specifications
| Catalog Number | D6430-PS4 |
| Brand | Zymo Research |
| Description | Quick-16S Plus NGS Library Prep Kit (V4) (96 preps) with Index Primer Set 4 |
| Target region | 16S rRNA V4 |
| Preps | 96 preps per kit |
| Primer sets | Index Primer Sets 1-4 available |
| Format | Premixed amplification plate |
| Hands-on time | About 30 minutes |
| Shipping Conditions | Ships on dry ice |
| Storage | -20C/4C |
| Note | Product contains multiple components shipped separately. (RT and Dry ice) |
Available Sizes & Components
- D6430-PS1 – Quick-16S Plus NGS Library Prep Kit (V4) (96 preps) with Index Primer Set 1
- D6430-PS2 – Quick-16S Plus NGS Library Prep Kit (V4) (96 preps) with Index Primer Set 2
- D6430-PS3 – Quick-16S Plus NGS Library Prep Kit (V4) (96 preps) with Index Primer Set 3
Applications
- 16S V4 amplicon sequencing
- High-throughput microbiome profiling
- Automated NGS library preparation
- Gut and environmental microbiome studies
