Product Description
Removing abundant double-stranded DNA while sparing single-stranded molecules opens up powerful normalization and detection methods. This Zymo Research Duplex-Specific Nuclease cuts dsDNA and the DNA strand in DNA-RNA hybrids, with virtually no activity toward ssDNA or RNA. That selectivity makes it a valuable tool for normalization and detection methods in transcriptomics and genomics.
This listing is for catalog number E1020-200: PureRec Duplex-Specific Nuclease, 1 U/µl, 200 µl. The enzyme can distinguish perfectly matched from mismatched short duplexes and needs at least 10 bp of dsDNA or a 15 bp perfectly matched DNA-RNA duplex to cleave. Activity depends on Mg2+, with 5 mM recommended for most uses. It is produced from a non-animal source, free of non-specific nucleases and RNases, and supplied as a 1 U/µl liquid in 200 µl or 1000 µl. LIGHT LABS ships genuine Zymo Research products with knowledgeable support from our Englewood, Colorado team.
Key Features
- Duplex selectivity – Digests dsDNA and DNA in DNA-RNA hybrids, sparing ssDNA and RNA.
- Mismatch discrimination – Distinguishes perfectly matched from mismatched short duplexes.
- Defined minimum substrate – Requires 10 bp dsDNA or a 15 bp matched DNA-RNA duplex.
- Non-animal source – Recombinantly produced and highly purified.
- Clean enzyme – Free of non-specific nucleases and RNases.
Specifications
| Catalog Number | E1020-200 |
| Brand | Zymo Research |
| Description | PureRec Duplex-Specific Nuclease, 1 U/µl, 200 µl |
| Concentration | 1 U/µl |
| Sizes | 200 µl; 1000 µl |
| Form | Liquid |
| Minimum substrate | 10 bp dsDNA or 15 bp perfectly matched DNA-RNA duplex |
| Cofactor | Mg2+ (5 mM recommended) |
| Source | Non-animal, recombinant |
| Shipping Conditions | Ships at room temperature |
| Storage | -20°C |
Available Sizes & Components
Applications
- cDNA library normalization
- Abundant transcript depletion
- SNP and mismatch detection
- microRNA detection assays
