Product Description
Bisulfite-converted DNA is notoriously hard to amplify cleanly, and nonspecific products can ruin methylation analysis. This Zymo Research hot-start polymerase is designed specifically for bisulfite-treated templates, producing specific amplicons with little or no by-product, while also performing well in conventional PCR. It suits epigenetics labs and general molecular biology alike.
This listing is for catalog number E2001: ZymoTaq DNA Polymerase, 50 Reactions. The heat-activated, thermostable enzyme stays inactive during setup, reducing primer dimers and off-target products; a 10-minute incubation at 95°C starts polymerization. All reagents for hot-start PCR are included. The enzyme also has 3'-terminal transferase activity, adding A overhangs that make products ready for TA cloning. Available in 50- and 200-reaction sizes. Add it to your LIGHT LABS order and keep your bench stocked.
Key Features
- Hot-start activation – Heat-activated enzyme reduces primer dimers and nonspecific products.
- Bisulfite PCR optimized – Designed for amplification of bisulfite-treated DNA.
- Specific amplicons – Generates targets with little or no by-product.
- TA-cloning ready – 3'-terminal transferase activity adds A overhangs.
- Complete reagents – Includes everything needed for hot-start PCR.
Specifications
| Catalog Number | E2001 |
| Brand | Zymo Research |
| Description | ZymoTaq DNA Polymerase, 50 Reactions |
| Sizes | 50 reactions; 200 reactions (5 U/µl, 80 µl) |
| Activation | 95°C for 10 minutes |
| Type | Heat-activated hot-start thermostable polymerase |
| Overhang | 3' A overhangs |
| Shipping Conditions | Ships on dry ice |
| Storage | -80°C |
Available Sizes & Components
Applications
- DNA methylation detection
- Bisulfite sequencing PCR
- Conventional PCR
- TA cloning
