Product Description
Analyzing methylation by high-resolution melt requires a qPCR mix that handles bisulfite-converted DNA and gives a strong, uniform signal. This Zymo Research premix supplies everything needed, combining a hot-start polymerase and an optimized buffer with sensitive dye-based detection. It is designed for epigenetics and general qPCR labs alike.
This listing is for catalog number E2054: ZymoTaq qPCR Premix, 50 Reactions. The mix includes SYTO 9, an intense dsDNA-specific fluorescent dye suited to real-time quantification and HRM analysis, along with a buffer system tailored for bisulfite-treated templates. It also performs general real-time PCR assays. The same technology underlies the Femto DNA Quantification Kits for low-copy detection. Available in 50- and 200-reaction sizes. LIGHT LABS ships genuine Zymo Research products with knowledgeable support from our Englewood, Colorado team.
Key Features
- Complete qPCR mix – Contains all reagents for quantitative PCR.
- Hot-start polymerase – Reduces nonspecific amplification.
- Bisulfite optimized – Buffer system tailored for bisulfite-treated DNA.
- SYTO 9 dye – Intense dsDNA-specific dye for sensitive detection.
- HRM compatible – Suited to high-resolution melt methylation analysis.
Specifications
| Catalog Number | E2054 |
| Brand | Zymo Research |
| Description | ZymoTaq qPCR Premix, 50 Reactions |
| Sizes | 50 reactions; 200 reactions |
| Dye | SYTO 9 |
| Polymerase | Hot-start |
| Optimized for | Bisulfite-treated DNA |
| Compatible analyses | qPCR, HRM |
| Shipping Conditions | Ships on dry ice |
| Storage | -80°C |
Available Sizes & Components
Applications
- High-resolution melt methylation analysis
- Methylation-specific qPCR
- General real-time PCR
- Low-copy DNA detection
