Product Description
Methylation studies need a fully methylated reference to calibrate assays and confirm bisulfite conversion. This Zymo Research CpG methylase methylates every cytosine in a CpG context in double-stranded DNA, whether the starting material is unmethylated or hemimethylated. It is a dependable way to generate fully methylated control DNA.
This listing is for catalog number E2011: CpG Methylase (M. Sssl), 400 U. The recombinant enzyme is purified from E. coli expressing the methyltransferase gene from Spiroplasma sp. strain MQ1. Reaction conditions are tuned to maximize processivity, giving rapid, complete and reproducible methylation. Each set includes 10X Reaction Buffer and S-adenosylmethionine cofactor, and is offered in 200 U and 400 U sizes. Add it to your LIGHT LABS order and keep your bench stocked.
Key Features
- Complete CpG methylation – Methylates all cytosines in 5'-CpG-3' sequences.
- Works on hemimethylated DNA – Accepts non-methylated and hemimethylated dsDNA.
- Recombinant enzyme – Derived from the Spiroplasma sp. MQ1 methyltransferase gene expressed in E. coli.
- Optimized processivity – Delivers rapid, reproducible methylation.
- Complete set – Supplied with 10X Reaction Buffer and SAM cofactor.
Specifications
| Catalog Number | E2011 |
| Brand | Zymo Research |
| Description | CpG Methylase (M. Sssl), 400 U |
| Sizes | 200 U; 400 U |
| Specificity | Cytosines in 5'...CpG...3' context |
| Substrate | Double-stranded non-methylated and hemimethylated DNA |
| Source | Recombinant, E. coli expressing Spiroplasma sp. MQ1 gene |
| Included | 10X Reaction Buffer, S-adenosylmethionine |
| Shipping Conditions | Ships on dry ice |
| Storage | -20°C |
Available Sizes & Components
Applications
- Methylated DNA control preparation
- Bisulfite conversion controls
- Methylation-specific PCR standards
- Epigenetics assay development
