Product Description
Fragmenting DNA without a sonicator saves equipment time and protects small samples. This Zymo Research endonuclease shears double-stranded DNA enzymatically, producing random-ended fragments of the size you need in a single step while keeping sample loss to a minimum. It offers a practical alternative to sonication for many workflows.
This listing is for catalog number E2018-200: dsDNA Shearase Plus, 200 U. The enzyme cleaves phosphodiester bonds to create fragments carrying 5'-phosphate and 3'-hydroxyl ends, with a strong preference for dsDNA. Because it works in low reaction volumes, it suits limited inputs where transfer losses matter. It is available in 50 U and 200 U sizes for library preparation and other fragmentation needs. LIGHT LABS stocks the Zymo Research line, so kits, buffers and refills are always within reach.
Key Features
- Enzymatic shearing – Fragments DNA without mechanical shearing equipment.
- dsDNA preference – Shows a strong preference for double-stranded DNA.
- Random ends – Produces random-ended fragments of the desired size.
- Defined termini – Yields 5'-phosphate and 3'-hydroxyl ends.
- Low-volume compatible – Minimizes sample loss with small inputs.
Specifications
| Catalog Number | E2018-200 |
| Brand | Zymo Research |
| Description | dsDNA Shearase Plus, 200 U |
| Sizes | 50 U; 200 U |
| Activity | Endonuclease with strong dsDNA preference |
| Ends produced | 5'-phosphate and 3'-hydroxyl |
| Procedure | Single step |
| Shipping Conditions | Ships on dry ice |
| Storage | -80°C |
Available Sizes & Components
Applications
- NGS library preparation
- DNA fragmentation for methylation analysis
- ChIP and MeDIP workflows
- Low-input DNA shearing
