Product Description
LC/MS quantification of global methylation works best when DNA is reduced to nucleosides. This Zymo Research nuclease mix digests DNA completely to individual nucleosides in a single step, delivering a faster, simpler workflow than traditional multi-enzyme protocols. It is designed for labs performing mass spectrometry methylation analysis.
This listing is for catalog number E2020: DNA Degradase Plus, 250 U. Because nucleosides lack the negatively charged phosphate group, they separate and ionize well for LC/MS analysis of whole-genome methylation such as 5-methylcytosine levels. The one-step digestion keeps preparation quick and consistent. It is available in 250 U and 1000 U sizes. The streamlined digestion improves consistency between samples. LIGHT LABS stocks the Zymo Research line, so kits, buffers and refills are always within reach.
Key Features
- Digests to nucleosides – Breaks DNA down to individual nucleosides.
- LC/MS ready – Phosphate-free products suit mass spectrometry analysis.
- One-step procedure – Faster and simpler than multi-step methods.
- Nuclease mix – Blended enzymes ensure complete degradation.
- Two sizes – Available in 250 U and 1000 U.
Specifications
| Catalog Number | E2020 |
| Brand | Zymo Research |
| Description | DNA Degradase Plus, 250 U |
| Sizes | 250 U; 1000 U |
| Product | Individual nucleosides |
| Procedure | One-step digestion |
| Downstream use | LC/MS |
| Shipping Conditions | Ships on dry ice |
| Storage | -80°C |
Available Sizes & Components
Applications
- Global DNA methylation by LC/MS
- 5-mC and 5-hmC quantification
- Nucleoside analysis
- Epigenetics research
