Product Description
Healthy, uniform cell growth before induction is key to consistent protein yields. Expansion Broth from Zymo Research is the growth stage of the Dual Media system, designed to expand E. coli cultures while keeping recombinant protein production in check. It is the first step in a two-stage protocol aimed at reliable, high-level protein production.
This listing is for catalog number M3012-100: Expansion Broth (EB), 100 mL. Grow cells in EB to build biomass, then transfer the culture to Overexpression Broth (OB) for high-level, consistent recombinant protein expression. Sold separately in 100 ml and 500 ml sizes, it lets labs restock the expansion medium independently to match their workflow. Using the matched EB and OB pair supports reproducible expression results. LIGHT LABS ships genuine Zymo Research products with knowledgeable support from our Englewood, Colorado team.
Key Features
- Growth-stage medium – Formulated for E. coli cell expansion.
- Pairs with OB – Used with Overexpression Broth for high-level expression.
- Consistent expression – Part of a two-stage system for reproducible results.
- Two sizes – Available in 100 ml and 500 ml.
Specifications
| Catalog Number | M3012-100 |
| Brand | Zymo Research |
| Description | Expansion Broth (EB), 100 mL |
| Sizes | 100 ml; 500 ml |
| Used with | Overexpression Broth (OB) |
| Shipping Conditions | Ships at room temperature |
| Storage | 4°C |
Available Sizes & Components
Applications
- E. coli culture expansion
- Recombinant protein expression
- Dual Media Set replenishment
- Protein production workflows
