MTC Bio OptiWell™ D3096-09 | 96-Well Deep Well Plate, Square V-Bottom, 0.5mL, Non-Sterile — 100/Case (10 Bags of 10)

The MTC Bio OptiWell™ D3096-09 is a 96-well deep well plate with square V-bottom wells and 0.5mL capacity per well — non-sterile, virgin medical-grade polypropylene, black printed alpha-numerics, minimal binding and liquid retention, compatible with all popular automated research platforms and robotic equipment including KingFisher™ and Accuris IsoPure™ magnetic bead platforms. OptiWell plates come in 24, 48, 96, or 384 format for virtually every research lab, HTS application, and automated/robotic platform. These plates are useful for bacterial cultures, centrifugation (up to 4,000 × g) and general sample storage. Plates are produced and packaged in a Class 100,000 clean room and are ANSI/SLAS 1-2004 compliant. All plates are certified non-pyrogenic and RNase/DNase free. Case of 100 plates — 10 bags of 10 plates each.

Square V-Bottom Wells — Maximum Sample Recovery in the Compact Deep Well Format The square V-bottom well geometry of the D3096-09 combines the maximum sample recovery advantage of a conical V-bottom with the space-maximizing square well cross-section — providing both complete sample concentration to the lowest point of each well and the maximum volume capacity within the ANSI/SLAS standard 96-well footprint. The V-bottom concentrates sample, beads, or pellets to the absolute lowest point of each well — minimizing dead volume and maximizing recovery by pipette tip or robotic probe. The square well cross-section provides a larger cross-sectional area than round wells of equivalent depth — delivering more usable well volume at the same plate height. For compound management, bead-based assay preparation, automated magnetic bead workflows, and small-volume sample processing where complete sample recovery from each well is the primary performance requirement, the square V-bottom geometry of the D3096-09 provides the optimal combination of recovery and volume efficiency.

0.5mL Per Well — Compact Format for Small-Volume 96-Well Deep Well Applications The 0.5mL per-well capacity of the D3096-09 sits between the standard microplate (0.2–0.3mL) and mid-range deep well formats — providing meaningful additional volume over standard microplates while maintaining the compact plate height that reduces instrument clearance requirements and storage footprint. For automated magnetic bead workflows, small-volume compound dilution series, sample reformatting from standard microplates to deep well format, and any application requiring more volume than a standard 96-well plate without the full depth of the 2.2mL format, the D3096-09 0.5mL square V-bottom is the correct specification.

D3096-09 vs. D3096-07 — Square V-Bottom vs. Round V-Bottom at Similar Volume:

D3096-09 in the Full OptiWell™ 96-Well Deep Well Family:

KingFisher™ and Accuris IsoPure™ Compatibility The D3096-09 is specifically noted for compatibility with KingFisher™ and Accuris IsoPure™ automated magnetic bead extraction platforms. At the 0.5mL volume, the square V-bottom geometry of the D3096-09 provides the correct well geometry for wash and elution steps in automated magnetic bead nucleic acid extraction protocols where smaller buffer volumes are used at specific workflow stages — complementing the larger 2.2mL D3096-43S H-style plate used for lysis and primary binding steps.

Automated Platform Compatibility — ANSI/SLAS 1-2004 Standard For automated research platforms and robotic equipment. The D3096-09 conforms to ANSI/SLAS 1-2004 standard 96-well microplate footprint dimensions — ensuring compatibility with all major automated liquid handling platforms including Hamilton, Tecan, Beckman Coulter, PerkinElmer, Agilent, KingFisher™, Accuris IsoPure™, and other standard HTS robotic systems.

4,000 × g Centrifugation Rating Centrifugation up to 4,000 × g. The robust polypropylene construction of the D3096-09 withstands centrifugal forces up to 4,000 × g — covering all standard plate centrifugation protocols used in bead collection, cell pelleting, and sample concentration at 0.5mL scale.

Virgin Medical-Grade Polypropylene — Minimal Binding and Liquid Retention Minimal binding / liquid retention. Virgin, medical-grade polypropylene. The medical-grade formulation provides minimal non-specific binding of biological molecules and minimal liquid retention — critical for maximizing sample recovery from the V-bottom well geometry.

Black Printed Alpha-Numerics — Instant Well Identification Black printed alpha-numerics. Molded black alpha-numeric well identification (A through H rows, 1 through 12 columns) allows instant identification of specific wells during liquid handling setup, plate reading, and data recording.

Class 100,000 Cleanroom Production — Certified Purity Plates are produced and packaged in a Class 100,000 clean room. All plates are certified non-pyrogenic and RNase/DNase free.

Compatible with Silicone Sealing Mats and Sealing Film The D3096-09 can be used with silicone sealing mats and various types of sealing film — compatible with all standard 96-well plate sealing formats.

Compatible Replacement — Cross-Reference Guide

The MTC Bio OptiWell™ D3096-09 is a direct compatible replacement for 96-well square V-bottom 0.5mL deep well plates from several major manufacturers:

• Thermo Fisher AB-0788 — Thermo Scientific 96-well square V-bottom deep well plate, 0.5mL
• Greiner Bio-One 786201 — 96-well deep well plate, square V-bottom, 0.5mL, polypropylene
• Eppendorf® 0030501012 — Eppendorf 96-well deepwell plate, square V-bottom, 0.5mL

Competitor brand names and catalog numbers are referenced for cross-referencing purposes only. Compatibility should be verified for your specific application and protocol.

Key Features

• MTC Bio OptiWell™ — Catalog # D3096-09
• 96-well deep well plate — square V-bottom wells
• 0.5mL per-well capacity
• Square V-bottom — maximum sample recovery and volume efficiency
• Compatible with KingFisher™ and Accuris IsoPure™ magnetic bead platforms
• Virgin medical-grade polypropylene — minimal binding and liquid retention
• Black printed alpha-numerics — instant well identification
• ANSI/SLAS 1-2004 compliant — compatible with all standard automated liquid handling platforms
• 4,000 × g centrifugation rating
• Compatible with silicone sealing mats and sealing film
• No lid, no skirt
• Non-sterile (autoclavable)
• Class 100,000 cleanroom production
• Certified RNase-free and DNase-free
• Certified non-pyrogenic
• Sigma-Aldrich catalog # MTCD309609
• Case of 100 plates — 10 bags of 10 plates

Supported Applications Automated magnetic bead nucleic acid extraction wash and elution steps on KingFisher™ and Accuris IsoPure™ platforms, compound dilution and preparation at 0.5mL scale, sample reformatting from standard microplates to deep well format, automated liquid handling sample processing, bead-based assay preparation at small volume, centrifugation workflows up to 4,000 × g, bacterial culture in 96-well format, general sample storage, and any 96-well application requiring a square V-bottom deep well format with 0.5mL per-well capacity and full automated platform compatibility.

Technical Specifications