Product Description
Purifying a His-tagged protein no longer has to take an afternoon. This Zymo Research spin-column kit captures up to 1 mg of His-tagged protein and delivers it in about five minutes, with elution in as little as 100 µl, so you can move from lysate to assay while your sample is still fresh.
This listing is for catalog number P2001: His-Spin Protein Miniprep, 10 Preps. The workflow is a simple bind, wash and elute sequence using a nickel affinity gel in a spin-column format, with dedicated binding, wash and elution buffers included. The resulting protein is highly pure and ready to use directly in enzymatic assays, biochemical characterization, SDS-PAGE and other sensitive downstream work. Kits are available in 10 and 50 prep sizes. Add it to your LIGHT LABS order and keep your bench stocked.
Key Features
- Five-minute purification – Spin, wash and elute workflow delivers results in minutes.
- Up to 1 mg capacity – Each prep can purify up to 1 mg of His-tagged protein.
- Concentrated elution – Recover protein in as little as 100 µl of His-Elution Buffer.
- High purity – Purified protein is ready for enzymatic assays and SDS-PAGE.
- Complete kit – Includes affinity gel, spin columns and matched buffers.
Specifications
| Catalog Number | P2001 |
| Brand | Zymo Research |
| Description | His-Spin Protein Miniprep, 10 Preps |
| Binding capacity | Up to 1 mg His-tagged protein per prep |
| Processing time | About 5 minutes |
| Elution volume | As little as 100 µl |
| Format | Spin column |
| Prep sizes | 10 preps; 50 preps |
| Shipping Conditions | Ships at room temperature |
| Storage | 4°C |
Available Sizes & Components
Applications
- His-tagged recombinant protein purification
- Enzyme activity assays
- SDS-PAGE and Western blot analysis
- Protein expression screening
- Biochemical characterization
