Product Description
Set up reverse transcription in under a minute at room temperature. This Zymo Research premix bundles everything needed for efficient, unbiased cDNA synthesis, so you only add RNA, making it a strong fit for two-step RT-qPCR studies that probe many targets from one sample. It keeps setup simple even for large experiments.
This listing is for catalog number R3012: ZymoScript RT Pre-Mix, 100 rxns. The ready-to-use mix contains random hexamer and oligo dT primers, dNTPs, RNase inhibitors, reverse transcriptase and an optimized buffer, and accepts from 0.1 pg up to 5 µg of input RNA. It performs well on GC-rich and structured templates. A blue tracking dye simplifies pipetting without interfering with fluorescent detection. Each kit provides 100 reactions. LIGHT LABS stocks the Zymo Research line, so kits, buffers and refills are always within reach.
Key Features
- All-in-one premix – primers, dNTPs, RNase inhibitors, enzyme and buffer in one tube.
- Sub-minute setup – simply add RNA at room temperature.
- Wide input range – synthesizes cDNA from 0.1 pg to 5 µg of RNA.
- Difficult templates – robust performance on GC-rich and highly structured RNA.
- Pipetting tracking dye – blue dye aids setup and does not affect fluorescent detection.
Specifications
| Catalog Number | R3012 |
| Brand | Zymo Research |
| Description | ZymoScript RT Pre-Mix, 100 rxns |
| RNA input | 0.1 pg to 5 µg |
| Primers | Random hexamer and oligo dT |
| Components | Primers, dNTPs, RNase inhibitors, reverse transcriptase, optimized buffer |
| Setup | Room temperature, under 1 minute |
| Kit size | 100 reactions |
| Shipping Conditions | Ships on dry ice |
| Storage | -80°C |
Applications
- Two-step RT-qPCR
- Multi-target gene expression analysis
- cDNA synthesis for PCR cloning
- Low-input RNA studies
