Product Description
Fast, reliable cloning starts with cells that are ready when you are. These Zymo Research premade Zymo 10B competent cells transform without heat shock: add DNA, mix and plate, with efficiencies greater than 10^8 transformants per µg pUC19. They are ready for routine cloning work.
This listing is for catalog number T3019: Mix & Go! Competent Cells - Zymo 10B, 10 x 100 µl. These chemically competent E. coli Zymo 10B cells are made by a method that removes heat shock and related handling from the protocol, so transformation takes just a mix and a spread. They are suitable for cloning, subcloning, PCR fragment cloning and library construction. Available as 10 x 100 µl tubes or 96 x 50 µl. Add it to your LIGHT LABS order and keep your bench stocked.
Key Features
- Heat-shock free – mix DNA with cells and spread directly onto solid medium.
- High efficiency – greater than 10^8 transformants per µg pUC19.
- Premade convenience – chemically competent cells arrive ready to transform.
- Cloning versatility – suited to cloning, subcloning, PCR fragment cloning and library work.
- Format options – supplied as 10 x 100 µl tubes or 96 x 50 µl.
Specifications
| Catalog Number | T3019 |
| Brand | Zymo Research |
| Description | Mix & Go! Competent Cells - Zymo 10B, 10 x 100 µl |
| Strain | E. coli Zymo 10B |
| Transformation efficiency | >10^8 transformants/µg pUC19 |
| Heat shock | Not required |
| Formats | 10 x 100 µl or 96 x 50 µl |
| Shipping Conditions | Ships on dry ice |
| Storage | -80°C |
Available Sizes & Components
Applications
- Plasmid cloning
- Subcloning
- PCR fragment cloning
- Library construction
