Product Description
Transformations do not get much simpler than this. These Zymo Research premade DH5 alpha cells need no heat shock or ice incubation sequence; just mix DNA with the cells and plate, and expect efficiencies above 10^8 transformants per µg pUC19 for everyday cloning. That makes them a practical everyday choice.
This listing is for catalog number T3007: Mix & Go! Competent Cells - DH5 Alpha, 10 x 100 µl. These chemically competent E. coli DH5 alpha cells are prepared by a method that removes heat shock from the protocol, so transformation is just a mix and a spread. They suit cloning, subcloning, PCR fragment cloning and library construction. Choose 10 x 100 µl tubes, 96 x 50 µl, or a 96 x 50 µl set with PCR plates and cover foils. Order it from LIGHT LABS and keep your workflow moving.
Key Features
- Heat-shock free – mix DNA with cells and spread directly onto solid medium.
- High efficiency – greater than 10^8 transformants per µg pUC19.
- Premade convenience – chemically competent cells arrive ready to transform.
- Cloning versatility – suited to cloning, subcloning, PCR fragment cloning and library work.
- Format options – supplied as 10 x 100 µl tubes, 96 x 50 µl, or 96 x 50 µl with 96-well PCR plates and cover foils.
Specifications
| Catalog Number | T3007 |
| Brand | Zymo Research |
| Description | Mix & Go! Competent Cells - DH5 Alpha, 10 x 100 µl |
| Strain | E. coli DH5 Alpha |
| Transformation efficiency | >10^8 transformants/µg pUC19 |
| Heat shock | Not required |
| Formats | 10 x 100 µl; 96 x 50 µl; 96 x 50 µl with 96-well PCR plates and cover foils |
| Shipping Conditions | Ships on dry ice |
| Storage | -80°C |
Available Sizes & Components
- T3009 – Mix & Go! Competent Cells - DH5 Alpha, 96 x 50 µl
- T3010 – Mix & Go! Competent Cells - DH5 Alpha, 96 x 50 µl
Applications
- Plasmid cloning
- Subcloning
- PCR fragment cloning
- Library construction
