Zymo Research Zymoclean™ Gel DNA Recovery Kit D4007 | Capped Columns, 50 Preps

Product Description

The Zymo Research Zymoclean™ Gel DNA Recovery Kit D4007 provides rapid purification of high-quality DNA from TAE/TBE-buffered agarose gels — in the 50-prep format with capped Zymo-Spin™ IC columns. Using Zymo-Spin™ column technology and a single-buffer system, the Zymoclean kit delivers ultra-pure, ready-to-use DNA in as little as 15 minutes — with no need for organic denaturants or chloroform. DNA is eluted in as little as 6 µL of low-salt elution buffer or water, yielding highly concentrated, sequencing-ready DNA across a wide fragment size range from ~50 bp to 23 kb. Eluted DNA is well-suited for DNA ligation, sequencing, labeling, PCR, and all standard downstream molecular biology applications. With over 2,100 citations in peer-reviewed literature, the Zymoclean is one of the most validated gel DNA recovery kits available.

D4007 vs. D4001 — Capped vs. Uncapped Columns: The Only Difference The D4007 and D4001 are identical kits in every way — the column cap is the sole difference:

The D4007 capped column format is the correct choice for labs that prefer capped columns — for easy labeling of individual columns during multi-sample processing, secure column storage between uses, and added sample security during centrifugation and handling. The column matrix, maximum binding capacity, buffer chemistry, and recovery performance are identical between both formats.

Capped Zymo-Spin™ IC Columns — Why Caps Matter The Zymo-Spin™ IC columns supplied with the D4007 feature a cap that covers the column top — providing three practical advantages over uncapped columns:

• Easy labeling — the flat cap surface provides a convenient area to write sample names, numbers, or identifiers directly on the column cap during multi-sample gel extraction workflows, eliminating sample mix-up risk
• Secure storage — capped columns can be stored between steps without risk of contamination or evaporation of the loaded sample or wash buffer from the column top
• Added sample security — the cap prevents accidental spills and contamination of the column contents during handling and centrifugation steps in busy laboratory environments

D4007 vs. D4008 — 50 Preps vs. 200 Preps:

Simple Single-Buffer Workflow — 15 Minutes The Zymoclean protocol is designed for speed and simplicity. Add the specially formulated Agarose Dissolving Buffer (ADB) directly to the excised gel slice, dissolve at 37–55°C, transfer to the Zymo-Spin™ IC Column, wash twice, and elute. The entire process requires only a single buffer addition before column loading — no ethanol precipitation, no organic extraction, no neutralization steps. From gel slice to purified DNA in 15 minutes.

Highly Concentrated Elution in Minimal Volume The Zymo-Spin™ IC column design permits DNA elution directly into as little as 6 µL of low-salt DNA Elution Buffer or water — yielding highly concentrated, sequencing-ready DNA from a minimal eluate volume. This is particularly valuable for downstream applications such as ligation reactions and next-generation sequencing library preparation, where high DNA concentration in a small volume is essential.

High Recovery Across a Wide Fragment Size Range The Zymoclean kit recovers DNA fragments from ~50 bp to 23 kb from TAE- and TBE-buffered agarose gels — covering the complete range of standard molecular biology fragment sizes from small PCR products and restriction fragments to large genomic DNA inserts. The single-buffer protocol and Zymo-Spin™ IC column matrix provide high, consistent recovery across this full size range without requiring protocol modifications for different fragment sizes.

Ultra-Pure DNA — Suited for All Downstream Applications Eluted DNA is well suited for use in DNA ligation, sequencing, labeling, PCR, etc. The Zymoclean purification process removes gel matrix, TAE/TBE buffer components, ethidium bromide, SYBR™ dyes, agarose impurities, and other inhibitors — yielding ultra-pure DNA with A260/280 ratios typically ≥1.8. The high purity of Zymoclean eluates makes them directly suitable for the most sensitive downstream applications including NGS library preparation, Sanger sequencing, isotope labeling, Gibson assembly, and ligation cloning — without further cleanup or precipitation.

Complete Zymoclean™ Gel DNA Recovery Kit Family:

Kit Contents — D4007 (50 Preps)

• Zymo-Spin™ IC Columns (Capped) — 50
• Collection Tubes — 50
• Agarose Dissolving Buffer (ADB)
• DNA Wash Buffer (concentrate — ethanol must be added prior to use)
• DNA Elution Buffer
• Instruction Manual

Key Features

• Zymo Research Zymoclean™ Gel DNA Recovery Kit — Catalog # D4007
• 50 preps — capped Zymo-Spin™ IC columns
• Rapid gel DNA extraction — 15 minutes from gel slice to purified DNA
• TAE and TBE-buffered agarose gel compatible
• Highly concentrated elution — ≥6 µL minimum elution volume
• Wide fragment size recovery — ~50 bp to 23 kb
• Ultra-pure DNA — suited for ligation, sequencing, labeling, PCR, NGS
• Single-buffer system — no organic solvents required
• Capped columns — easy labeling, secure storage, sample security
• Over 2,100 citations in peer-reviewed literature
• 200-prep capped format: D4008
• Uncapped column format (50 preps): D4001

Supported Applications Gel extraction of PCR products for sequencing, gel purification of restriction digest fragments for cloning, DNA fragment isolation for ligation reactions, agarose gel DNA recovery for NGS library preparation, size-selection of DNA fragments by gel electrophoresis followed by column purification, removal of primers and unincorporated nucleotides from PCR reactions via gel separation and recovery, isolation of specific DNA bands from multiplex PCR, gel purification of DNA labeling reactions, and any application requiring purification of specific DNA fragments from TAE- or TBE-buffered agarose gels in the ~50 bp to 23 kb size range.

Technical Specifications