Product Description
LIGHT LABS is proud to offer the Zymo Research Zymoclean™ Gel DNA Recovery Kit with Capped Columns (Cat # D4008) — the high-volume 200-prep format of Zymo Research's widely used gel DNA extraction kit, supplied with capped Zymo-Spin™ IC columns. The capped column format is preferred by labs requiring secure, leak-proof column handling, or where vacuum manifold compatibility with a sealed cap is needed. Ideal for core facilities, high-throughput research groups, and any lab running frequent gel DNA extraction workflows at scale.
The Zymoclean™ Gel DNA Recovery Kit provides rapid purification of high-quality DNA from TAE or TBE-buffered agarose gels in just minutes. Using Zymo Research's ADB (Agarose Dissolving Buffer), the gel slice is rapidly dissolved and the DNA is bound directly to the Zymo-Spin™ IC column without the need for phenol/chloroform or organic solvents. The result is ultra-pure DNA (A260/280 ≥ 1.8, A260/230 ≥ 1.8) in minimal elution volumes (≥ 6 µl) — highly concentrated and immediately ready for ligation, sequencing, labeling, PCR, restriction digestion, and NGS applications. LIGHT LABS is your trusted source for Zymo Research products.
KEY FEATURES
- Rapid gel DNA extraction in just minutes — no phenol/chloroform or organic solvents required
- Capped Zymo-Spin™ IC columns — secure, leak-proof design for easy handling and vacuum manifold compatibility
- 200-prep high-volume format — ideal for high-throughput labs and core facilities
- ADB (Agarose Dissolving Buffer) rapidly dissolves gel slice for direct DNA binding to column
- DNA size range: 50 bp to 23 kb
- Up to 5 µg DNA binding capacity per column
- DNA recovery: 70–80% for 50 bp to 10 kb; 40–60% for 11 kb to 23 kb
- High DNA purity — A260/280 ≥ 1.8, A260/230 ≥ 1.8
- Minimum elution volume: ≥ 6 µl for highly concentrated DNA eluates
- Compatible with TAE and TBE buffered agarose gels
- Maximum gel input: up to 400 mg agarose per column
- Eluted DNA immediately ready for ligation, sequencing, labeling, PCR, restriction endonuclease digestion, and NGS library preparation
- Fisher Scientific Catalog No. 50-444-181
- Genesee Scientific Catalog No. 11-301C
- Made in the USA by Zymo Research
SPECIFICATIONS
| Attribute | Detail |
|---|---|
| Catalog Number | D4008 |
| Fisher Scientific Catalog No. | 50-444-181 |
| Genesee Scientific Catalog No. | 11-301C |
| Brand | Zymo Research |
| Kit Name | Zymoclean™ Gel DNA Recovery Kit |
| Column Type | Zymo-Spin™ IC (Capped) |
| Number of Preps | 200 |
| DNA Binding Capacity | Up to 5 µg per column |
| DNA Size Range | 50 bp to 23 kb |
| DNA Recovery | 70–80% (50 bp – 10 kb); 40–60% (11 kb – 23 kb) |
| DNA Purity | A260/280 ≥ 1.8; A260/230 ≥ 1.8 |
| Minimum Elution Volume | ≥ 6 µl |
| Maximum Gel Input | Up to 400 mg agarose per column |
| Gel Buffer Compatibility | TAE and TBE buffered agarose gels |
| Equipment Required | Microcentrifuge |
| Storage Temperature | Room temperature |
| Kit Components | ADB Agarose Dissolving Buffer (2 x 100 mL), DNA Wash Buffer concentrate (24 mL), DNA Elution Buffer (4 mL), Zymo-Spin™ IC Capped Columns (200), Collection Tubes (200), Instruction Manual |
APPLICATIONS
- Gel DNA extraction and band recovery from preparative agarose gels
- PCR product size selection and gel purification
- Restriction endonuclease digestion product isolation from gels
- DNA ligation insert and vector preparation from gels
- NGS library preparation gel size selection and cleanup
- DNA sequencing template preparation from gels
- DNA labeling reaction product purification from gels
- Cloning workflow gel band isolation
- Any application requiring rapid, high-quality DNA recovery from TAE/TBE agarose gels
ORDERING INFORMATION
| Cat # | Description | Column Type | Capacity | Preps |
|---|---|---|---|---|
| D4008 | Zymoclean™ Gel DNA Recovery Kit | Capped (IC) | 5 µg | 200 |
| D4007 | Zymoclean™ Gel DNA Recovery Kit | Capped (IC) | 5 µg | 50 |
| D4002 | Zymoclean™ Gel DNA Recovery Kit | Uncapped (I) | 5 µg | 200 |
| D4001 | Zymoclean™ Gel DNA Recovery Kit | Uncapped (I) | 5 µg | 50 |
| D4001T | Zymoclean™ Gel DNA Recovery Kit | Uncapped (I) | 5 µg | 10 |
| D4046 | Zymoclean™ Large Fragment Gel DNA Recovery Kit | Uncapped (IC-XL) | 10 µg | 100 |
| D4045 | Zymoclean™ Large Fragment Gel DNA Recovery Kit | Uncapped (IC-XL) | 10 µg | 25 |
| D4022 | ZR-96 Zymoclean™ Gel DNA Recovery Kit | 96-Well Plate | 5 µg/well | 4 x 96 |
| D4021 | ZR-96 Zymoclean™ Gel DNA Recovery Kit | 96-Well Plate | 5 µg/well | 2 x 96 |
Product Videos
Custom Field
Product Reviews
Write a Review
×