Product Description
LIGHT LABS is proud to offer the Zymo Research ZymoPURE™ II Plasmid Maxiprep Kit (Cat # D4203) — the fastest and highest-yielding plasmid maxiprep kit available, delivering up to 3 mg of highly concentrated, EndoZero™ vaccine-grade, transfection-ready plasmid DNA from up to 150 mL of E. coli overnight culture in under 18 minutes — with no ethanol or alcohol precipitation step and no slow, cumbersome gravity flow column required.
Utilizing Zymo Research's patented modified alkaline lysis system combined with a patented ZymoPURE™ binding chemistry and Zymo-Spin™ V-PX Column technology, the D4203 produces plasmid DNA at remarkable concentrations of up to 6 µg/µL eluted directly from a spin column — ready for immediate use without any post-purification processing steps. The EndoZero™ technology achieves endotoxin levels at or below 0.025 EU/µg — vaccine-grade purity proven suitable for transfection of even the most sensitive primary cell lines, CRISPR delivery, lentiviral vector production, in vivo studies, and other demanding applications where endotoxin control is paramount.
As an added convenience, the ZymoPURE™ II Maxiprep kit features color-coded buffers (including a signature pink/purple binding buffer color change) that provide easy visual confirmation of complete bacterial cell lysis and neutralization — minimizing errors and making the kit accessible to both novice and experienced researchers. Users have reported DNA yields 10x greater than leading conventional maxiprep kits. LIGHT LABS is your trusted source for Zymo Research plasmid purification products.
KEY FEATURES
- Fastest maxiprep available — complete protocol in under 18 minutes from culture to purified plasmid DNA
- Highest yield in its class — up to 3 mg of plasmid DNA per prep from up to 150 mL E. coli culture
- Ultra-concentrated eluate — up to 6 µg/µL eluted directly from spin column using a microcentrifuge; minimum elution volume ≥ 200 µl
- No ethanol or alcohol precipitation step required — eliminates the most time-consuming step of conventional maxiprep protocols
- No gravity flow column — vacuum or centrifuge-based binding for dramatically faster, more convenient processing
- EndoZero™ vaccine-grade purity — endotoxin levels ≤0.025 EU/µg; suitable for sensitive primary cell transfection, in vivo studies, and viral vector production
- Patented ZymoPURE™ binding chemistry combined with Zymo-Spin™ V-PX Column — higher yield and purity vs. all conventional maxiprep technologies
- Color-coded buffers for easy, error-free visual confirmation of complete bacterial cell lysis and neutralization
- Plasmid DNA free of endotoxins, salt, protein, and RNA — ready for immediate use in demanding downstream applications
- Compatible with both vacuum manifold and centrifuge processing — maximum lab flexibility
- DNA purity: A260/280 ≥ 1.8; A260/230 ≥ 2.0
- Plasmid size range: up to 200 kb
- Fisher Scientific Catalog No. NC1121573
- Genesee Scientific Catalog No. 11-555B
- Made in the USA by Zymo Research
SPECIFICATIONS
| Attribute | Detail |
|---|---|
| Catalog Number | D4203 |
| Fisher Scientific Catalog No. | NC1121573 |
| Brand | Zymo Research |
| Kit Name | ZymoPURE™ II Plasmid Maxiprep Kit |
| Number of Preps | 20 |
| Maximum DNA Yield | Up to 3 mg per prep |
| Maximum DNA Concentration | Up to 6 µg/µL |
| Minimum Elution Volume | ≥ 200 µl |
| Maximum Culture Volume | Up to 150 mL overnight E. coli culture |
| Endotoxin Level | ≤0.025 EU/µg (EndoZero™ — vaccine grade) |
| DNA Purity | A260/280 ≥ 1.8; A260/230 ≥ 2.0 |
| Plasmid Size Range | Up to 200 kb |
| Processing Time | Less than 18 minutes |
| Ethanol/Alcohol Precipitation | Not required |
| Gravity Flow Column | Not required |
| Processing Method | Vacuum manifold or centrifuge |
| Column Type | Zymo-Spin™ V-PX Column with 15 mL Reservoir-X |
| Buffer System | Color-coded buffers for visual lysis/neutralization verification |
| Storage Temperature | Room temperature (ZymoPURE™ P1: 4–8°C after RNase A addition) |
| Shelf Life | 1 year from date of purchase |
| Kit Components | ZymoPURE™ P1, P2, P3 Buffers (color-coded); ZymoPURE™ Binding Buffer; ZymoPURE™ Wash 1 & 2; Elution Buffer; Zymo-Spin™ V-PX Column Assemblies; 15 mL Reservoir-X; 50 mL Conical Tubes; Collection Tubes; EndoZero™ Spin-Columns |
APPLICATIONS
- Large-scale plasmid DNA purification for cell transfection
- Endotoxin-sensitive primary cell and stem cell transfection requiring vaccine-grade DNA
- Viral vector (lentiviral, AAV, adenoviral) plasmid DNA production
- CRISPR plasmid DNA preparation for in vitro and in vivo delivery
- In vivo gene delivery and DNA vaccination studies
- Synthetic biology and genome editing workflows
- Restriction endonuclease digestion and mapping
- DNA sequencing of large plasmid constructs (up to 200 kb)
- Bacterial transformation and cloning
- PCR amplification and template preparation
- Any application requiring large quantities of the highest-purity, endotoxin-free plasmid DNA
ORDERING INFORMATION
| Cat # | Description | Max Yield | Preps |
|---|---|---|---|
| D4203 | ZymoPURE™ II Plasmid Maxiprep Kit | Up to 3 mg | 20 |
| D4204 | ZymoPURE™ II Plasmid Maxiprep Kit | Up to 3 mg | 50 |
| D4201 | ZymoPURE™ II Plasmid Midiprep Kit | Up to 400 µg | 50 |
| D4200 | ZymoPURE™ II Plasmid Midiprep Kit | Up to 400 µg | 25 |
| D4209 | ZymoPURE™ II Plasmid Gigaprep Kit | Up to 10 mg | 4 |
| D4027 | Zyppy™ Plasmid Maxiprep Kit | Up to 500 µg | 10 |
| D4028 | Zyppy™ Plasmid Maxiprep Kit | Up to 500 µg | 20 |
| D4025 | Zyppy™ Plasmid Midiprep Kit | Up to 200 µg | 25 |
| D4026 | Zyppy™ Plasmid Midiprep Kit | Up to 200 µg | 50 |
