Product Description
LIGHT LABS is proud to offer the Zymo Research ZymoPURE™ II Plasmid Midiprep Kit (Cat # D4201) — widely recognized as the fastest and highest-yielding midiprep kit available, now in the best-value 50-prep format. The ZymoPURE™ II Midiprep delivers up to 400 µg of highly concentrated, EndoZero™ vaccine-grade, transfection-ready plasmid DNA from up to 50 mL of E. coli overnight culture in less than 20 minutes — with no ethanol precipitation step and no slow, cumbersome gravity flow column required.
Utilizing Zymo Research's patented modified alkaline lysis system combined with a novel ZymoPURE™ binding chemistry, the D4201 produces plasmid DNA at remarkable concentrations of up to 3 µg/µL eluted directly from a spin column using a standard microcentrifuge. The EndoZero™ technology couples ZymoPURE's proprietary binding chemistry with innovative EndoZero™ II Spin-Columns to achieve endotoxin levels at or below 0.025 EU/µg — vaccine-grade purity suitable for transfection of even the most sensitive primary cell lines, in vivo studies, viral vector production, and other demanding applications where endotoxin control is critical. The plasmid DNA is bound onto the column using either a vacuum or centrifuge, providing maximum flexibility for any lab setup.
The 50-prep D4201 format provides the best per-prep value in the ZymoPURE™ II Midiprep line, making it ideal for labs with frequent large-scale plasmid DNA needs. LIGHT LABS is your trusted source for Zymo Research plasmid purification products.
KEY FEATURES
- Fastest midiprep available — complete protocol in under 20 minutes from culture to purified plasmid DNA
- Highest yield in its class — up to 400 µg of plasmid DNA per prep from up to 50 mL E. coli culture
- Ultra-concentrated eluate — up to 3 µg/µL eluted directly from spin column using a microcentrifuge
- No ethanol precipitation step required — eliminates the most time-consuming step of conventional midiprep protocols
- No gravity flow column — vacuum or centrifuge-based binding for faster, more convenient processing
- EndoZero™ vaccine-grade purity — endotoxin levels ≤0.025 EU/µg; suitable for sensitive primary cell transfection, in vivo studies, and viral vector production
- EndoZero™ II Spin-Columns included for superior endotoxin removal performance
- Innovative ZymoPURE™ binding chemistry — novel column binding system enables higher yield and purity vs. conventional midiprep technologies
- Compatible with both vacuum manifold and centrifuge processing — maximum lab flexibility
- 50-prep format — best per-prep value in the ZymoPURE™ II Midiprep line
- Genesee Scientific Catalog No. 11-550B
- Made in the USA by Zymo Research
SPECIFICATIONS
| Attribute | Detail |
|---|---|
| Catalog Number | D4201 |
| Brand | Zymo Research |
| Kit Name | ZymoPURE™ II Plasmid Midiprep Kit |
| Number of Preps | 50 |
| Maximum DNA Yield | Up to 400 µg per prep |
| Maximum DNA Concentration | Up to 3 µg/µL |
| Maximum Culture Volume | Up to 50 mL overnight E. coli culture |
| Endotoxin Level | ≤0.025 EU/µg (EndoZero™ — vaccine grade) |
| Processing Time | Less than 20 minutes |
| Ethanol Precipitation | Not required |
| Gravity Flow Column | Not required |
| Processing Method | Vacuum manifold or centrifuge |
| Column Type | Zymo-Spin™ III-P with EndoZero™ II Spin-Column |
| Storage Temperature | Room temperature (ZymoPURE™ P1: 4–8°C after RNase A addition) |
| Shelf Life | 1 year from date of purchase |
| Kit Components | ZymoPURE™ P1, P2, P3 Buffers; ZymoPURE™ Binding Buffer; ZymoPURE™ Wash 1 & 2; Elution Buffer; Zymo-Spin™ III-P Column Assemblies; EndoZero™ II Spin-Columns; Conical Reservoirs; Collection Tubes |
APPLICATIONS
- Large-scale plasmid DNA purification for cell transfection
- Endotoxin-sensitive primary cell transfection requiring vaccine-grade DNA
- Viral vector (lentiviral, AAV, adenoviral) plasmid DNA production
- CRISPR plasmid DNA preparation for in vitro and in vivo delivery
- In vivo gene delivery and DNA vaccination studies
- Restriction endonuclease digestion and mapping
- DNA sequencing of large plasmid constructs
- Bacterial transformation and cloning
- PCR amplification and template preparation
- Plasmid-mediated gene silencing
- Any application requiring large quantities of ultra-pure, endotoxin-free plasmid DNA
ORDERING INFORMATION
| Cat # | Description | Max Yield | Preps |
|---|---|---|---|
| D4201 | ZymoPURE™ II Plasmid Midiprep Kit | Up to 400 µg | 50 |
| D4200 | ZymoPURE™ II Plasmid Midiprep Kit | Up to 400 µg | 25 |
| D4203 | ZymoPURE™ II Plasmid Maxiprep Kit | Up to 800 µg | 25 |
| D4204 | ZymoPURE™ II Plasmid Maxiprep Kit | Up to 800 µg | 50 |
| D4209 | ZymoPURE™ II Plasmid Gigaprep Kit | Up to 10 mg | 4 |
| D4027 | Zyppy™ Plasmid Maxiprep Kit | Up to 500 µg | 10 |
| D4028 | Zyppy™ Plasmid Maxiprep Kit | Up to 500 µg | 20 |
| D4025 | Zyppy™ Plasmid Midiprep Kit | Up to 200 µg | 25 |
| D4026 | Zyppy™ Plasmid Midiprep Kit | Up to 200 µg | 50 |
