Product Description
When 0.22 µm is not the right cut, these bottle top vacuum filters give you 0.1 µm and 0.45 µm options for tighter retention or faster clarification. Pick a PES membrane for low protein binding and high flow, or a PVDF membrane for broad chemical resistance, in sizes from 50 mL to 1000 mL.
This listing is for NEST catalog number 342012: Bottle Top Vacuum Filter, 250 mL, 0.1 µm PES Membrane, Sterile, Individually Bagged, Case of 24 (24 x 1). Each unit combines a hydrophilic membrane (50 mm diameter on 50 and 150 mL units, 89 mm on 250 to 1000 mL units) with a virgin USP Class IV polystyrene cup and cap and an ABS neck. Units are molded and packed in an ISO 7 cleanroom in a cGMP, ISO 13485:2016 and ISO 9001 facility, individually bagged and E-beam sterilized to SAL 10⁻⁶. Lots are tested free of pyrogens, cytotoxins, DNase, RNase, DNA and BSE/TSE, and are certified free of animal products. LIGHT LABS stocks the NEST Scientific line and ships quickly from our Englewood, Colorado team.
Key Features
- Two Membrane Choices – PES for low protein binding and high flow, PVDF for wide chemical compatibility
- Multiple Pore Sizes – 0.1 µm for fine retention and 0.45 µm for clarification and prefiltration
- Low Extractables – Virgin USP Class IV polystyrene cup keeps leachables to a minimum
- Large Filter Area – 89 mm membrane on 250 to 1000 mL units for higher throughput
- Individually Bagged Sterile – E-beam sterilized to SAL 10⁻⁶ and opened only when needed
- Lot-Tested Purity – Free of pyrogens, cytotoxins, DNase/RNase, DNA and BSE/TSE
Specifications
| Catalog Number | 342012 |
| Brand | NEST Scientific |
| Description | Bottle Top Vacuum Filter, 250 mL, 0.1 µm PES Membrane, Sterile, Individually Bagged |
| Sold As | Case of 24 (24 x 1) |
| Volume | 250 mL |
| Membrane | Polyethersulfone (PES) |
| Pore Size | 0.1 µm |
| Membrane Diameter | 89 mm |
| Neck / Receiver | 45 mm neck |
| Sterile | Yes |
| Units per Pack | 1 |
| Units per Case | 24 |
| Type | Bottle top vacuum filter (filter unit only) |
| Housing | Virgin USP Class IV polystyrene cup and cap, ABS neck |
| Max. Working Temperature | 45 °C |
| Chemical Limits | Not for chloroform, esters, amides, strong acids or strong bases |
Available Options
- 347012 – Bottle Top Vacuum Filter, 50 mL, 0.1 µm PES Membrane, Sterile, Individually Bagged, Case of 24 (24 x 1)
- 347013 – Bottle Top Vacuum Filter, 50 mL, 0.45 µm PES Membrane, Sterile, Individually Bagged, Case of 24 (24 x 1)
- 347112 – Bottle Top Vacuum Filter, 50 mL, 0.1 µm PVDF Membrane, Sterile, Individually Bagged, Case of 24 (24 x 1)
- 347113 – Bottle Top Vacuum Filter, 50 mL, 0.45 µm PVDF Membrane, Sterile, Individually Bagged, Case of 24 (24 x 1)
- 346012 – Bottle Top Vacuum Filter, 150 mL, 0.1 µm PES Membrane, Sterile, Individually Bagged, Case of 24 (24 x 1)
- 346013 – Bottle Top Vacuum Filter, 150 mL, 0.45 µm PES Membrane, Sterile, Individually Bagged, Case of 24 (24 x 1)
- 346112 – Bottle Top Vacuum Filter, 150 mL, 0.1 µm PVDF Membrane, Sterile, Individually Bagged, Case of 24 (24 x 1)
- 346113 – Bottle Top Vacuum Filter, 150 mL, 0.45 µm PVDF Membrane, Sterile, Individually Bagged, Case of 24 (24 x 1)
- 342013 – Bottle Top Vacuum Filter, 250 mL, 0.45 µm PES Membrane, Sterile, Individually Bagged, Case of 24 (24 x 1)
- 342112 – Bottle Top Vacuum Filter, 250 mL, 0.1 µm PVDF Membrane, Sterile, Individually Bagged, Case of 24 (24 x 1)
- 342113 – Bottle Top Vacuum Filter, 250 mL, 0.45 µm PVDF Membrane, Sterile, Individually Bagged, Case of 24 (24 x 1)
- 343012 – Bottle Top Vacuum Filter, 500 mL, 0.1 µm PES Membrane, Sterile, Individually Bagged, Case of 24 (24 x 1)
- 343013 – Bottle Top Vacuum Filter, 500 mL, 0.45 µm PES Membrane, Sterile, Individually Bagged, Case of 24 (24 x 1)
- 343112 – Bottle Top Vacuum Filter, 500 mL, 0.1 µm PVDF Membrane, Sterile, Individually Bagged, Case of 24 (24 x 1)
Applications
- Mycoplasma-reducing media filtration (0.1 µm)
- Media and buffer clarification (0.45 µm)
- Prefiltration before sterile filtering
- HPLC and GC sample preparation
- Cell culture solution preparation
