Product Description
Reliable detection of DNA methylation starts with an antibody that ignores everything except 5-methylcytosine. This mouse monoclonal, clone 7D21, recognizes methylated cytosines in single-stranded DNA with no detectable cross-reactivity to unmethylated or hydroxymethylated cytosine, giving clear, specific signal in methylation enrichment and immunoassays. It is a practical tool for labs mapping methylation patterns.
This listing is for catalog number A3002-15: Anti-5-Methylcytosine (Clone 7D21), 0.5 µg/µl. The antibody is supplied at 0.5 µg/µl and is well suited to methylated DNA immunoprecipitation (MeDIP), ELISA and dot blot formats. Denature your DNA, bind the antibody to methylated fragments, and capture or detect the complexes with your preferred secondary reagents. Several vial sizes, from 15 µl up to 200 µl, let you match quantity to project scale. Add it to your LIGHT LABS order and keep your bench stocked.
Key Features
- Clone 7D21 monoclonal – Mouse monoclonal antibody that targets 5-mC.
- High specificity – No detectable cross-reactivity with unmodified or 5-hmC cytosines.
- Single-stranded DNA binding – Recognizes 5-mC in denatured DNA.
- Multiple assay formats – Suitable for MeDIP, ELISA and dot blot.
- Range of sizes – Offered in 15, 30, 50 and 200 µl vials.
Specifications
| Catalog Number | A3002-15 |
| Brand | Zymo Research |
| Description | Anti-5-Methylcytosine (Clone 7D21), 0.5 µg/µl |
| Host / clonality | Mouse monoclonal, clone 7D21 |
| Target | 5-methylcytosine in single-stranded DNA |
| Concentration | 0.5 µg/µl |
| Validated uses | MeDIP, ELISA, dot blot |
| Pack sizes | 15 µl; 30 µl; 50 µl; 200 µl |
| Shipping Conditions | Ships on dry ice |
| Storage | -20°C |
Available Sizes & Components
- A3002-200 – Anti-5-Methylcytosine (Clone 7D21), 0.5 µg/µl
- A3002-30 – Anti-5-Methylcytosine (Clone 7D21), 0.5 µg/µl
- A3002-50 – Anti-5-Methylcytosine (Clone 7D21), 0.5 µg/µl
Applications
- Methylated DNA immunoprecipitation (MeDIP)
- Global DNA methylation ELISA
- Dot blot detection of 5-mC
- Epigenetics research
