Product Description
Quantitative methylation assays need a positive reference with a known, complete level of modification. This standard provides DNA in which cytosines are present as 5-methylcytosine, making it a dependable top point for calibrating antibody-based, enzymatic, and other methods that measure cytosine modifications. It can be used alone or as part of the matched standard series.
This listing is for catalog number D5405-2: 5-Methylcytosine DNA Standard, 2 µg. It is the methylated member of the 5-Methylcytosine & 5-Hydroxymethylcytosine DNA Standard Set, sharing its sequence with the unmodified and hydroxymethylated standards so that results reflect modification alone. The standard is supplied as 2 µg and can be combined with the unmodified standard to create intermediate calibration points. LIGHT LABS stocks the Zymo Research line, so kits, buffers and refills are always within reach.
Key Features
- Methylated reference – Cytosines are present as 5-methylcytosine.
- Matched sequence – Shares its sequence with the cytosine and 5-hmC standards.
- Calibration use – Serves as a positive reference in quantitative assays.
- 2 µg supply – Provided as 2 µg of DNA.
Specifications
| Catalog Number | D5405-2 |
| Brand | Zymo Research |
| Description | 5-Methylcytosine DNA Standard, 2 µg |
| Modification | 5-methylcytosine |
| Quantity | 2 µg |
| Set | Component of D5405 |
| Shipping Conditions | Ships on blue ice |
| Storage | -20°C |
Applications
- 5-mC assay calibration
- ELISA positive control
- Antibody specificity testing
- Methylation method validation
