Product Description
Large DNA fragments are notoriously hard to pull out of agarose, but this Zymo Research kit streamlines the job. It purifies and concentrates high-quality, large-sized DNA from gel slices in about 15 minutes, using a spin column designed for efficient recovery of bigger fragments. It is ideal for cloning larger inserts.
This listing is for catalog number D4046: Zymoclean Large Fragment DNA Recovery Kit, 100 Preps. Add Agarose Dissolving Buffer to the gel slice, let it dissolve and transfer the mixture to the supplied Zymo-Spin IC-XL column. DNA binds, is washed and is eluted without organic denaturants or chloroform. The recovered DNA is ideal for PCR, sequencing, restriction digestion and ligation. Available in 25- and 100-prep sizes. LIGHT LABS stocks the Zymo Research line, so kits, buffers and refills are always within reach.
Key Features
- Large fragment recovery – Optimized for purifying large-sized DNA from gels.
- Fast procedure – Typically completed in about 15 minutes.
- Organic-free – No chloroform or organic denaturants required.
- Specialized column – Zymo-Spin IC-XL column supports efficient recovery.
- Downstream ready – Suited to PCR, sequencing, digestion and ligation.
Specifications
| Catalog Number | D4046 |
| Brand | Zymo Research |
| Description | Zymoclean Large Fragment DNA Recovery Kit, 100 Preps |
| Processing time | About 15 minutes |
| Column | Zymo-Spin IC-XL |
| Format | 25 or 100 preps |
| Dissolving buffer | Agarose Dissolving Buffer (ADB) |
| Shipping Conditions | Ships at room temperature |
| Storage | Room Temperature |
Available Sizes & Components
Applications
- Large insert cloning
- Vector backbone recovery
- Sequencing templates
- Restriction digestion and ligation
