Product Description
LIGHT LABS is proud to offer the Zymo Research Zymoclean™ Large Fragment DNA Recovery Kit (Cat # D4045) — a specialized, streamlined kit engineered for the rapid recovery of high-quality, large-sized DNA fragments from both high and low-melting point agarose gels. While standard gel extraction kits are limited to fragments of 10 kb or less, the D4045 is specifically designed to recover DNA fragments ranging from ~50 bp all the way up to 200 kb — making it the ideal solution for researchers working with genomic DNA fragments, BAC/PAC plasmids, viral and phage DNA, and other high-molecular-weight DNA preparations.
The proprietary Agarose Dissolving Buffer (ADB) rapidly dissolves the gel slice without the need for organic denaturants or chloroform, and the large-format Zymo-Spin™ IC-XL column technology delivers high-quality, purified DNA in just 15 minutes. Eluted DNA is ultra-pure and immediately ready for use in endonuclease digestion, sequencing, ligation, PCR, labeling, and NGS applications. LIGHT LABS is your trusted source for Zymo Research products.
KEY FEATURES
- Specifically designed for recovery of large-sized DNA fragments from agarose gels — up to 200 kb
- Broad size range: ~50 bp to >200 kb — far exceeding the range of standard gel extraction kits
- Compatible with both high and low-melting point agarose gels in TAE or TBE buffer
- Fast 15-minute protocol — simple dissolve-bind-wash-elute workflow
- No organic denaturants or chloroform required — clean, safe, straightforward procedure
- Proprietary Agarose Dissolving Buffer (ADB) rapidly dissolves gel slice for efficient DNA binding
- Large-format Zymo-Spin™ IC-XL column for gentle, high-capacity large fragment recovery
- Up to 10 µg DNA binding capacity per column
- Low volume elution (≥ 10 µl) allows for highly concentrated DNA eluates
- High-quality purified DNA — ideal for sequencing, ligation, endonuclease digestion, PCR, labeling, and NGS
- Genesee Scientific Catalog No. 11-301L
- Stored at room temperature
- Made in the USA by Zymo Research
SPECIFICATIONS
| Attribute | Detail |
|---|---|
| Catalog Number | D4045 |
| Genesee Scientific Catalog No. | 11-301L |
| Brand | Zymo Research |
| Kit Name | Zymoclean™ Large Fragment DNA Recovery Kit |
| Format | Spin Column (Zymo-Spin™ IC-XL) |
| Number of Preps | 25 |
| DNA Binding Capacity | Up to 10 µg per column |
| DNA Size Range | ~50 bp to >200 kb |
| Gel Compatibility | High and low-melting point agarose (TAE or TBE) |
| Processing Time | ~15 minutes |
| Minimum Elution Volume | ≥ 10 µl |
| Storage Temperature | Room temperature |
| Kit Components | Agarose Dissolving Buffer / ADB (50 mL), DNA Wash Buffer concentrate (6 mL), DNA Elution Buffer (1 mL), Zymo-Spin™ IC-XL Columns (25), Collection Tubes (50), Instruction Manual |
APPLICATIONS
- Recovery of large DNA fragments (>10 kb, up to 200 kb) from agarose gels
- Genomic DNA fragment isolation for cloning and library construction
- BAC/PAC and large plasmid DNA recovery from gels
- Viral and phage DNA gel extraction
- Long-range PCR product purification from gels
- Preparative gel electrophoresis DNA recovery
- NGS library preparation from gel-purified large fragments
- Any application requiring gel extraction of high-molecular-weight DNA
ORDERING INFORMATION
| Cat # | Description | Size Range | Capacity | Preps |
|---|---|---|---|---|
| D4045 | Zymoclean™ Large Fragment DNA Recovery Kit | ~50 bp – >200 kb | 10 µg | 25 |
| D4046 | Zymoclean™ Large Fragment DNA Recovery Kit | ~50 bp – >200 kb | 10 µg | 100 |
| D4001 | Zymoclean™ Gel DNA Recovery Kit (standard, uncapped columns) | 50 bp – 23 kb | 5 µg | 50 |
| D4002 | Zymoclean™ Gel DNA Recovery Kit (standard, uncapped columns) | 50 bp – 23 kb | 5 µg | 200 |
| D4007 | Zymoclean™ Gel DNA Recovery Kit (capped columns) | 50 bp – 23 kb | 5 µg | 50 |
| D4008 | Zymoclean™ Gel DNA Recovery Kit (capped columns) | 50 bp – 23 kb | 5 µg | 200 |
| D4021 | ZR-96 Zymoclean™ Gel DNA Recovery Kit (96-well) | 50 bp – 23 kb | 5 µg/well | 2 x 96 |
| D4022 | ZR-96 Zymoclean™ Gel DNA Recovery Kit (96-well) | 50 bp – 23 kb | 5 µg/well | 4 x 96 |
