Product Description
Stop spending sequencing reads on host DNA. This kit strips away contaminating eukaryotic DNA before purification, so more of your data comes from the microbes you care about; in saliva, human DNA can drop from 65% to under 1% of the total. It is well suited to saliva and tissue samples.
This listing is for catalog number D4310: HostZERO Microbial DNA Kit, 50 Preps. Host cells are selectively lysed and their released DNA enzymatically degraded, leaving intact microbial cells. Total DNA is then purified with ZymoBIOMICS unbiased lysis and isolation chemistry, capturing DNA from living microbes while preserving accurate community representation. The workflow is designed to recover high amounts of bacterial DNA for metagenomic sequencing and other microbial analyses. LIGHT LABS ships genuine Zymo Research products with knowledgeable support from our Englewood, Colorado team.
Key Features
- Host DNA depletion – Selectively lyses eukaryotic cells and degrades their DNA.
- Dramatic host reduction – Human DNA in saliva reduced from 65% to less than 1%.
- Live-microbe focus – Captures DNA from intact, living microbial cells.
- Unbiased purification – Built on ZymoBIOMICS non-biased DNA isolation.
- High bacterial recovery – Designed to maximize bacterial DNA yield.
Specifications
| Catalog Number | D4310 |
| Brand | Zymo Research |
| Description | HostZERO Microbial DNA Kit, 50 Preps |
| Host DNA reduction (saliva) | From 65% to less than 1% human DNA |
| Method | Selective eukaryotic lysis and host DNA degradation, then total DNA purification |
| Pack size | 50 preps |
| Shipping Conditions | Ships on dry ice |
| Storage | -20°C |
| Note | Product contains multiple components packaged separately |
Applications
- Shotgun metagenomic sequencing
- Saliva and oral microbiome studies
- Tissue-associated microbiome research
- Pathogen detection research
- 16S rRNA gene sequencing
