Product Description
Reliable methylation data starts with knowing exactly how fully methylated and fully unmethylated DNA behave in your assay. This human control set gives you both references, so each bisulfite run can be checked against a known positive and a known negative before results from precious samples are trusted.
This listing is for catalog number D5014: Human Methylated & Non-methylated DNA Set, DNA w/ Primers. The non-methylated standard is genomic DNA from HCT116 DKO cells, a line carrying knockouts of both the DNMT1 and DNMT3b methyltransferases. The methylated standard is the same DNA after enzymatic methylation at CpG sites. A dedicated primer set is included, and the controls are intended for use with the EZ DNA Methylation family of bisulfite conversion products. Add it to your LIGHT LABS order and keep your bench stocked.
Key Features
- Positive and negative controls – Provides CpG-methylated and non-methylated human DNA in one set.
- DNMT double knockout source – Non-methylated DNA comes from HCT116 cells lacking DNMT1 and DNMT3b.
- Enzymatically methylated – The methylated standard is generated by in vitro CpG methylation of the same DNA.
- Primer set included – Specially designed primers support PCR-based conversion checks.
- Set or single items – Methylated and non-methylated DNAs are also listed as separate catalog items.
Specifications
| Catalog Number | D5014 |
| Brand | Zymo Research |
| Description | Human Methylated & Non-methylated DNA Set, DNA w/ Primers |
| Source | HCT116 DKO (DNMT1-/-, DNMT3b-/-) genomic DNA |
| Components | Methylated DNA, non-methylated DNA, primers |
| Individual DNAs | 5 µg/20 µl each (D5014-1, D5014-2) |
| Compatible kits | EZ DNA Methylation family |
| Shipping Conditions | Ships on blue ice |
| Storage | -20°C |
Available Sizes & Components
- D5014-1 – Human HCT116 DKO Non-Methylated DNA, 5 µg/20 µl
- D5014-2 – Human HCT116 DKO Methylated DNA, 5 µg/20 µl
Applications
- Bisulfite conversion efficiency testing
- Methylation-specific PCR controls
- Bisulfite sequencing quality control
- Methylation assay development
