Product Description
Each step of a methylated DNA immunoprecipitation can lose material, so a built-in tracer that reports enrichment is invaluable. This control combines methylated and unmethylated plasmid in a defined ratio, letting you verify that the pulldown enriched methylated DNA as expected before committing samples to sequencing or arrays.
This listing is for catalog number D5101-2: Methylated/Non-methylated Control DNA & Primer Set. The control is a 1:4 mixture of fully methylated pUC19 (pUC19m) and non-methylated pUC19. The methylated plasmid carries a mutation that creates an Nco I site absent from the unmethylated form, so Nco I digestion distinguishes the two species after amplification. Matching control primers are supplied for monitoring the stages of the Methylated-DNA IP workflow. LIGHT LABS stocks the Zymo Research line, so kits, buffers and refills are always within reach.
Key Features
- Defined ratio – Methylated and non-methylated pUC19 are mixed at 1:4.
- Nco I discrimination – A unique Nco I site in pUC19m separates the two forms.
- Primers included – Control primers amplify the plasmid for monitoring.
- Step-by-step tracking – Used to follow the different stages of the MeDIP procedure.
Specifications
| Catalog Number | D5101-2 |
| Brand | Zymo Research |
| Description | Methylated/Non-methylated Control DNA & Primer Set |
| Composition | Methylated pUC19 : non-methylated pUC19 at 1:4 |
| Discrimination | Nco I site unique to methylated pUC19m |
| Included | Control DNA and control primers |
| For use with | Methylated-DNA IP Kit |
| Shipping Conditions | Ships on blue ice |
| Storage | -20°C |
Applications
- MeDIP enrichment monitoring
- Methylated-DNA IP Kit replenishment
- Immunoprecipitation troubleshooting
