Product Description
Troubleshooting a methylation PCR becomes much simpler when you can separate amplification problems from conversion problems. This pre-converted human control lets you do exactly that, giving you a template that has already been through bisulfite treatment so primers, polymerase, and cycling conditions can be validated on their own.
This listing is for catalog number D5015: Bisulfite Converted Universal Methylated Human DNA Standard. The DNA matches the Universal Methylated Human DNA Standard but has already been bisulfite-converted with the EZ DNA Methylation-Direct kit (D5020). It is supplied at 20 ng/µl with enough material for 50 reactions, together with a validated primer set that amplifies a segment of the converted template. Use it with PCR, methylation-specific PCR, and other amplification-based assays. LIGHT LABS stocks the Zymo Research line, so kits, buffers and refills are always within reach.
Key Features
- Pre-converted template – Already bisulfite-treated, so it isolates downstream PCR performance from conversion.
- Validated primers – The included primer set is designed and tested to amplify the converted DNA.
- Ready concentration – Supplied as a 20 ng/µl solution for straightforward reaction setup.
- 50 reactions – Provides enough control material for 50 reactions.
- Fully methylated origin – Derived from the Universal Methylated Human DNA Standard.
Specifications
| Catalog Number | D5015 |
| Brand | Zymo Research |
| Description | Bisulfite Converted Universal Methylated Human DNA Standard |
| Concentration | 20 ng/µl |
| Quantity | Sufficient for 50 reactions |
| Conversion method | EZ DNA Methylation-Direct (D5020) |
| Included | Converted DNA and validated primer set |
| Shipping Conditions | Ships on blue ice |
| Storage | -20°C |
Applications
- MSP positive control
- Bisulfite PCR optimization
- Primer validation for converted DNA
- Amplification-based methylation assay controls
