Product Description
Some methylation experiments demand a baseline with no methylation at all, and bacterial DNA from the right strain delivers exactly that. This genomic DNA gives you a clean negative reference, helping you confirm that signals in your assay reflect genuine methylation rather than background or nonspecific detection.
This listing is for catalog number D5016: E. coli Non-Methylated Genomic DNA, 5 µg/20 µl. The DNA is isolated from E. coli strain ER2925, which lacks both Dam and Dcm methyltransferase activity, so the adenine and cytosine marks normally found in E. coli DNA are absent. It is supplied as 5 µg in 20 µl. Include it as a negative control in restriction-based, antibody-based, and other methylation analyses that require completely unmodified DNA. Add it to your LIGHT LABS order and keep your bench stocked.
Key Features
- Dam-/Dcm- strain – Isolated from E. coli ER2925, which lacks both common bacterial methylases.
- Negative control – Provides methylation-free DNA for baseline measurements.
- Genomic DNA format – Supplied as purified genomic DNA ready for assay use.
- Defined quantity – Provided as 5 µg in 20 µl.
Specifications
| Catalog Number | D5016 |
| Brand | Zymo Research |
| Description | E. coli Non-Methylated Genomic DNA, 5 µg/20 µl |
| Strain | E. coli ER2925 (Dam-, Dcm-) |
| Quantity | 5 µg/20 µl |
| Role | Negative control for methylation analysis |
| Shipping Conditions | Ships on blue ice |
| Storage | -20°C |
Applications
- Negative control for methylation assays
- Methylation-sensitive restriction digestion
- Baseline for 5-mC ELISA and MeDIP
- Assay specificity testing
