Product Description
Removing nonspecifically bound chromatin starts with a well-defined first wash. This replacement buffer performs the initial wash of Protein A bead, antibody, and chromatin complexes in the Zymo-Spin ChIP Kit, helping lower background before the subsequent washes and making it easy to restock just this component.
This listing is for catalog number D5210-3-30: Chromatin Wash Buffer I, 30 mL. Apply the buffer to the immunoprecipitated complexes as the first of three sequential washes in the Zymo-Spin ChIP protocol, followed by Chromatin Wash Buffers II and III. It is the same reagent included in the Zymo-Spin ChIP Kit (D5209, D5210), is supplied as 30 ml, and is stored at room temperature. Add it to your LIGHT LABS order and keep your bench stocked.
Key Features
- First wash – Performs the initial wash of bead, antibody, and chromatin complexes.
- Background reduction – Helps remove nonspecifically bound material.
- Kit-matched component – Identical to the buffer in the Zymo-Spin ChIP Kit.
- Room temperature storage – Supplied as 30 ml with no refrigeration needed.
Specifications
| Catalog Number | D5210-3-30 |
| Brand | Zymo Research |
| Description | Chromatin Wash Buffer I, 30 mL |
| Volume | 30 ml |
| Storage | Room temperature |
| Wash step | First of three |
| For use with | Zymo-Spin ChIP Kit (D5209, D5210) |
| Shipping Conditions | Ships at room temperature |
| Storage | Room Temperature |
Applications
- ChIP bead washing
- Zymo-Spin ChIP Kit replenishment
- ChIP-seq sample preparation
