Product Description
Releasing chromatin from beads efficiently helps determine how much immunoprecipitated DNA you ultimately recover. This concentrated elution buffer is the replacement component for the Zymo-Spin ChIP Kit, helping you finish additional ChIP preparations without purchasing an entire new kit or changing a validated protocol. It is a practical choice for labs that run ChIP regularly.
This listing is for catalog number D5210-6-10: 5X Chromatin Elution Buffer, 10 mL. Dilute the 5X concentrate as described in the Zymo-Spin ChIP protocol and use it to elute chromatin from the washed Protein A bead and antibody complexes before reverse cross-linking and DNA purification. It is the same buffer supplied in the complete kit and is packaged as a 10 ml volume. LIGHT LABS stocks the Zymo Research line, so kits, buffers and refills are always within reach.
Key Features
- Chromatin elution – Releases immunoprecipitated chromatin from bead complexes.
- 5X concentrate – Diluted to working strength before use.
- Kit-matched component – Same buffer supplied in the Zymo-Spin ChIP Kit.
- 10 ml volume – Convenient size for restocking.
Specifications
| Catalog Number | D5210-6-10 |
| Brand | Zymo Research |
| Description | 5X Chromatin Elution Buffer, 10 mL |
| Concentration | 5X |
| Volume | 10 ml |
| For use with | Zymo-Spin ChIP Kit |
| Shipping Conditions | Ships at room temperature |
| Storage | Room Temperature |
Applications
- ChIP chromatin elution
- Zymo-Spin ChIP Kit replenishment
- ChIP-seq sample preparation
