Product Description
Finishing the wash sequence properly prepares immunoprecipitated chromatin for elution with minimal carryover. This replacement buffer carries out the third wash of Protein A bead, antibody, and chromatin complexes in the Zymo-Spin ChIP Kit, letting labs replenish individual components as needed instead of buying a full kit.
This listing is for catalog number D5210-5-30: Chromatin Wash Buffer III, 30 mL. Apply it after Chromatin Wash Buffers I and II, then proceed to elution with the 5X Chromatin Elution Buffer as described in the Zymo-Spin ChIP protocol. It matches the kit component used in D5209 and D5210, is supplied as 30 ml, and is stored at room temperature. Order it from LIGHT LABS and keep your workflow moving.
Key Features
- Third wash – Completes the wash series for bead, antibody, and chromatin complexes.
- Elution ready – Prepares complexes for the chromatin elution step.
- Kit-matched component – Identical to the buffer in the Zymo-Spin ChIP Kit.
- Room temperature storage – Supplied as 30 ml with no refrigeration needed.
Specifications
| Catalog Number | D5210-5-30 |
| Brand | Zymo Research |
| Description | Chromatin Wash Buffer III, 30 mL |
| Volume | 30 ml |
| Storage | Room temperature |
| Wash step | Third of three |
| For use with | Zymo-Spin ChIP Kit (D5209, D5210) |
| Shipping Conditions | Ships at room temperature |
| Storage | Room Temperature |
Applications
- ChIP bead washing
- Zymo-Spin ChIP Kit replenishment
- ChIP-qPCR sample preparation
