Product Description
Few enzymes are as useful for probing chromatin structure as one that digests exposed linker DNA while leaving nucleosome-protected DNA behind. This nuclease cleaves both single- and double-stranded DNA and RNA, making it a versatile tool for nucleosome mapping as well as general nucleic acid removal.
This listing is for catalog number D5220-1: Micrococcal Nuclease, 10 U/100 µ. Complete digestion yields mono- and oligonucleotides carrying 3′-phosphate ends. In chromatin work, controlled partial digestion of isolated nuclei releases nucleosome-protected fragments for downstream study. The enzyme is supplied at 10 U in 100 µl and is the same nuclease included in the EZ Nucleosomal DNA Prep Kit, which also offers matched digestion and stop buffers. Order it from LIGHT LABS and keep your workflow moving.
Key Features
- Broad substrate range – Cleaves single- and double-stranded DNA and RNA.
- Defined products – Complete digestion yields mono- and oligonucleotides with 3′-phosphates.
- Chromatin mapping – Well suited to releasing nucleosome-protected DNA.
- Kit-matched enzyme – Same nuclease supplied in the EZ Nucleosomal DNA Prep Kit.
Specifications
| Catalog Number | D5220-1 |
| Brand | Zymo Research |
| Description | Micrococcal Nuclease, 10 U/100 µ |
| Substrates | Single- and double-stranded DNA and RNA |
| Products | Mono- and oligonucleotides with 3′-phosphates |
| Quantity | 10 U/100 µl |
| Shipping Conditions | Ships on dry ice |
| Storage | -80°C |
Applications
- Nucleosome mapping
- MNase-seq
- Chromatin accessibility studies
- Nucleic acid removal from lysates
