Product Description
Stopping a nuclease reaction at the right moment is what separates well-defined nucleosomal fragments from over-digested DNA. This concentrated stop buffer halts micrococcal nuclease activity in the EZ Nucleosomal DNA Prep Kit and readies the sample for DNA purification in a single addition. It is available separately to extend opened kits.
This listing is for catalog number D5220-4: 5X MN Stop Buffer, 6 mL. Add the 5X buffer to the digestion reaction at the end of the incubation, as directed in the EZ Nucleosomal DNA Prep protocol, then proceed to spin-column purification of the nucleosome-associated DNA. It matches the kit component and is supplied in a 6 ml volume for convenient restocking. Use it together with MN Digestion Buffer. Order it from LIGHT LABS and keep your workflow moving.
Key Features
- Reaction stop – Ends micrococcal nuclease digestion.
- Purification ready – Prepares samples for nucleosomal DNA cleanup.
- 5X concentrate – Added directly to the digestion reaction.
- Kit-matched component – Same buffer supplied in the EZ Nucleosomal DNA Prep Kit.
Specifications
| Catalog Number | D5220-4 |
| Brand | Zymo Research |
| Description | 5X MN Stop Buffer, 6 mL |
| Concentration | 5X |
| Volume | 6 ml |
| For use with | EZ Nucleosomal DNA Prep Kit |
| Shipping Conditions | Ships at room temperature |
| Storage | Room Temperature |
Applications
- MNase digestion control
- Nucleosomal DNA preparation
- EZ Nucleosomal DNA Prep Kit replenishment
