Product Description
Mapping 5-hydroxymethylcytosine at single-base resolution usually means complex chemistry, but this library prep kit avoids bisulfite treatment altogether. The result is genome-wide 5-hmC profiling from as little as 100 ng of DNA, with lower sequencing depth requirements and simpler bioinformatics than bisulfite-based approaches typically demand.
This listing is for catalog number D5450: RRHP 5-hmC Library Prep Kit, 12 Preps. Reduced Representation Hydroxymethylation Profiling relies on glucosylation of 5-hmC within MspI sites, which protects those sites from MspI cleavage. Fragments lacking protected 5-hmC at the adapter junction are cut and fail to amplify, so only 5-hmC-containing fragments are sequenced, and read frequency tracks 5-hmC level. The kit includes the reagents needed and comes in 12- and 25-prep sizes. LIGHT LABS ships genuine Zymo Research products with knowledgeable support from our Englewood, Colorado team.
Key Features
- Bisulfite free – Profiles 5-hmC without bisulfite conversion.
- Single-base resolution – Identifies 5-hmC positions within MspI sites.
- Low input – Works with DNA inputs as low as 100 ng.
- Efficient sequencing – Requires lower sequencing depth than bisulfite methods.
- Simple analysis – Read counts correlate with 5-hmC levels for straightforward bioinformatics.
Specifications
| Catalog Number | D5450 |
| Brand | Zymo Research |
| Description | RRHP 5-hmC Library Prep Kit, 12 Preps |
| Minimum input | 100 ng DNA |
| Resolution | Single base |
| Bisulfite | Not required |
| Principle | Glucosylation of 5-hmC blocks MspI digestion |
| Kit sizes | 12 (D5450), 25 (D5451) preps |
| Shipping Conditions | Ships on dry ice |
| Storage | Room Temperature/-80C |
Available Sizes & Components
Applications
- Genome-wide 5-hmC profiling
- Neuroepigenetics research
- Hydroxymethylation biomarker discovery
- Comparative 5-hmC studies across tissues
