Product Description
Fragmentomics studies depend on preserving the exact ends of every DNA molecule, and this single-stranded library kit is built to do just that. It prepares sequencing libraries from genomic, cell-free, and FFPE-derived DNA without end repair, keeping native fragment termini intact for high-fidelity analysis of fragmentation patterns.
This listing is for catalog number D5464: Zymo-Seq SPLAT DNA Library Kit, 12 Preps. Splinted Ligation Adapter Tagging works on single-stranded DNA. First, unique splinted adapters are ligated to the DNA concurrently; second, libraries are amplified by PCR with unique dual indexes. Pre-fragmented inputs from 10 ng to 500 ng are supported, and sequencing-ready libraries can be produced in as little as 3 hours. The kit contains 12 preps. Order it from LIGHT LABS and keep your workflow moving.
Key Features
- Native ends preserved – Direct adapter ligation keeps all original nucleotides at fragment termini.
- No end repair – Eliminates the end-repair step entirely.
- Versatile inputs – Accepts genomic, cell-free, and FFPE-derived DNA.
- Two-step workflow – Ligation followed by indexing PCR delivers libraries in about 3 hours.
- Unique dual indexes – Supports multiplexed sequencing.
Specifications
| Catalog Number | D5464 |
| Brand | Zymo Research |
| Description | Zymo-Seq SPLAT DNA Library Kit, 12 Preps |
| Input | 10–500 ng pre-fragmented DNA |
| Sample types | Genomic DNA, cfDNA, FFPE-derived DNA |
| Time | As little as 3 hours |
| Indexing | Unique dual indexes |
| Preps | 12 |
| Shipping Conditions | Ships on dry ice |
| Storage | -80C, -20C, 4C |
| Note | Product contains multiple components shipped separately. (RT and Dry ice) |
Applications
- cfDNA fragmentomics
- Liquid biopsy research
- FFPE DNA sequencing
- Damaged or degraded DNA libraries
