Product Description
Liquid biopsy and archival samples often contain short, nicked DNA that standard library methods throw away. This whole-genome bisulfite sequencing kit builds high-quality methyl-seq libraries from cell-free DNA, FFPE DNA, and genomic DNA, starting from as little as 5 ng of cfDNA or fragmented DNA.
This listing is for catalog number D5463: Zymo-Seq Trio WGBS Library Kit, 96 Preps. The workflow has three steps: gentle bisulfite conversion, direct ligation of Illumina-compatible splinted adapters onto fragments of any size, and index PCR. Skipping second-strand synthesis, end repair, and dA-tailing reduces bias and keeps native methylation at fragment ends intact. Open-source bioinformatic tools and a guide support co-detection of genetic information. Kits contain 24 or 96 preps. LIGHT LABS ships genuine Zymo Research products with knowledgeable support from our Englewood, Colorado team.
Key Features
- Low input – Builds libraries from as little as 5 ng of cfDNA or fragmented DNA.
- Diverse samples – Works with cfDNA, FFPE DNA, and genomic DNA.
- Splinted adapters – Ligate directly onto DNA fragments of any size, including nicked or damaged ones.
- Reduced bias – Eliminates second-strand synthesis, end repair, and dA-tailing.
- Analysis support – Validated open-source tools enable co-detection of genetic information.
Specifications
| Catalog Number | D5463 |
| Brand | Zymo Research |
| Description | Zymo-Seq Trio WGBS Library Kit, 96 Preps |
| Sample types | cfDNA, FFPE DNA, genomic DNA |
| Minimum input | 5 ng cfDNA or fragmented DNA |
| Workflow | Bisulfite conversion, splinted adapter ligation, index PCR |
| Platform | Illumina-compatible |
| Kit sizes | 24 (D5462), 96 (D5463) preps |
| Shipping Conditions | Ships on dry ice |
| Storage | -80C/-20C/4C/Room Temperature |
| Note | Product contains multiple components shipped separately |
Available Sizes & Components
Applications
- Cell-free DNA methylation profiling
- Liquid biopsy research
- FFPE methylome sequencing
- Methylation biomarker discovery
