Product Description
Whole-genome bisulfite sequencing gives base-level methylation calls across every cytosine, and this kit makes that comprehensive approach far less laborious. Completing library construction in a single tube removes the separate fragmentation, enzymatic, and cleanup steps of ligation-based methods, cutting hands-on time and supporting higher throughput.
This listing is for catalog number D5465: Zymo-Seq WGBS Library Kit, 24 Reactions. Intact genomic DNA is bisulfite converted first. In one tube, the converted DNA then undergoes second-strand synthesis, adapter addition by tagmentation, and amplification with indexing. After purification, libraries are ready for Illumina sequencing. The kit works with any species for CG, CHG, and CHH methylation calling and detects more than 90% of human and mouse CpG sites. It contains 24 reactions. LIGHT LABS stocks the Zymo Research line, so kits, buffers and refills are always within reach.
Key Features
- Single-tube workflow – Library preparation steps after conversion occur in one tube.
- Tagmentation based – Removes fragmentation, enzymatic, and cleanup steps.
- High CpG coverage – Detects over 90% of human and mouse CpG sites.
- All cytosine contexts – Supports CG, CHG, and CHH methylation calling in any species.
- Illumina ready – Purified libraries are ready for sequencing.
Specifications
| Catalog Number | D5465 |
| Brand | Zymo Research |
| Description | Zymo-Seq WGBS Library Kit, 24 Reactions |
| Input | Intact genomic DNA |
| Workflow | Bisulfite conversion, then single-tube second-strand synthesis, tagmentation, indexing PCR |
| Coverage | Over 90% of human and mouse CpG sites |
| Contexts | CG, CHG, CHH |
| Platform | Illumina |
| Reactions | 24 |
| Shipping Conditions | Ships on dry ice |
| Storage | -80C/-20C/Room Temperature |
Applications
- Whole-methylome sequencing
- Plant methylation in CHG and CHH contexts
- Developmental and cancer epigenetics
- Higher-throughput WGBS projects
