Product Description
Single-nucleotide methylation data from the most informative regions of the genome can be obtained at a fraction of the cost of whole-genome sequencing. This all-in-one library kit simplifies the classic reduced representation bisulfite sequencing method, making it practical for high-throughput screening and comparison experiments. Libraries are compatible with standard bisulfite sequencing analysis.
This listing is for catalog number D5461: Zymo-Seq RRBS Library Kit, 48 Preps. RRBS focuses sequencing on CpG-rich regions by restriction digestion before bisulfite conversion and library amplification. With human genomic DNA, libraries cover at least 70% of CpG islands, promoters, and gene bodies, providing broad coverage of regulatory regions while greatly reducing sequencing requirements. The kit supplies the reagents for library construction in 24- or 48-prep sizes. LIGHT LABS stocks the Zymo Research line, so kits, buffers and refills are always within reach.
Key Features
- All-in-one kit – Provides a simplified RRBS library workflow.
- Single-nucleotide resolution – Profiles methylation base by base in CpG-rich regions.
- Broad coverage – Covers at least 70% of human CpG islands, promoters, and gene bodies.
- Cost efficient – Reduces sequencing cost compared with whole-genome approaches.
- Two kit sizes – Offered as 24 or 48 preps.
Specifications
| Catalog Number | D5461 |
| Brand | Zymo Research |
| Description | Zymo-Seq RRBS Library Kit, 48 Preps |
| Coverage | At least 70% of CpG islands, promoters, and gene bodies (human gDNA) |
| Resolution | Single nucleotide |
| Kit sizes | 24 (D5460), 48 (D5461) preps |
| Shipping Conditions | Ships on dry ice |
| Storage | -80C/-20C/Room Temperature |
| Note | Product contains multiple components shipped separately. (RT and Dry ice) |
Available Sizes & Components
Applications
- High-throughput methylation screening
- Case-control methylation comparisons
- CpG island and promoter methylation profiling
- Cancer epigenetics research
