Product Description
Microbiome results are only meaningful if you know how much bias your workflow introduces. This mock community from Zymo Research gives labs a known starting point, letting them check whether DNA extraction, library prep and analysis faithfully reproduce a defined mix of bacteria and yeasts before trusting results from real samples.
This listing is for catalog number D6300: ZymoBIOMICS Microbial Community Standard, 10 Preps. The standard is a cellular mixture of ten organisms: three easy-to-lyse Gram-negative bacteria, five tough-to-lyse Gram-positive bacteria and two tough-to-lyse yeasts. Because it enters the workflow at the very first step, it exposes lysis bias and downstream errors alike. Use it to build and optimize new pipelines or run it routinely as a positive control alongside study samples. Each kit supplies 10 preps. Add it to your LIGHT LABS order and keep your bench stocked.
Key Features
- Defined composition – Ten microbes at known theoretical abundances based on genomic DNA.
- Lysis challenge – Mixes easy-to-lyse Gram-negatives with tough Gram-positives and yeasts.
- Whole-workflow control – Enters at extraction to reveal bias from collection to analysis.
- Pipeline optimization – Guides construction and tuning of new microbiomics workflows.
- Routine QC – Serves as an ongoing positive control for sequencing runs.
Specifications
| Catalog Number | D6300 |
| Brand | Zymo Research |
| Description | ZymoBIOMICS Microbial Community Standard, 10 Preps |
| Format | Cellular mock community |
| Preps | 10 preps |
| Organisms | 8 bacteria (3 Gram-negative, 5 Gram-positive) and 2 yeasts |
| Theoretical composition (gDNA) | L. monocytogenes, P. aeruginosa, B. subtilis, E. coli, S. enterica, L. fermentum, E. faecalis, S. aureus at 12% each; S. cerevisiae and C. neoformans at 2% each |
| Shipping Conditions | Ships at room temperature |
| Storage | -80°C |
Applications
- Validating DNA extraction methods
- 16S rRNA and shotgun metagenomic QC
- Bioinformatics pipeline benchmarking
- Microbiome workflow optimization
- Routine positive control
