Product Description
Relative abundance alone cannot tell you how many microbes are actually in a sample. This Zymo Research spike-in control adds an in-sample reference that supports absolute cell number quantification while also flagging extraction bias, giving high-biomass microbiome studies a more complete picture. It is well suited to stool and other samples rich in microbes.
This listing is for catalog number D6320-10: ZymoBIOMICS Spike-in Control I (High Microbial Load), 250 Preps. The control contains equal cell numbers of two bacterial strains: Imtechella halotolerans, which is Gram-negative, and Allobacillus halotolerans, which is Gram-positive. Spiked directly into each sample before extraction, the pair acts as an in situ positive control whose recovery reveals lysis bias. Accurately quantified cell counts allow conversion of sequencing reads to absolute numbers. Available in 25- and 250-prep sizes. Order it from LIGHT LABS and keep your workflow moving.
Key Features
- In situ positive control – Spiked directly into each sample for NGS-based microbiome measurements.
- Absolute quantification – Accurately counted cells enable conversion of reads to cell numbers.
- Bias detection – Gram-negative and Gram-positive strains reveal differences in lysis efficiency.
- Equal cell ratio – Two strains present at equal cell numbers.
- Two pack sizes – Offered in 25-prep and 250-prep formats.
Specifications
| Catalog Number | D6320-10 |
| Brand | Zymo Research |
| Description | ZymoBIOMICS Spike-in Control I (High Microbial Load), 250 Preps |
| Organisms | Imtechella halotolerans (Gram-negative), Allobacillus halotolerans (Gram-positive) |
| Ratio | Equal cell numbers |
| Sample load | High microbial load |
| Pack sizes | 25 preps; 250 preps |
| Shipping Conditions | Ships at room temperature |
| Storage | -80°C |
Available Sizes & Components
Applications
- Absolute microbial load quantification
- High-biomass microbiome samples such as stool
- Extraction bias monitoring
- NGS microbiome QC
- Comparative microbiome studies
