Product Description
Large 16S rRNA sequencing projects get bogged down by bead clean-ups, gel checks and separate quantification steps. This Zymo Research library prep kit streamlines the whole process, cutting hands-on time while addressing common 16S pitfalls such as PCR chimera formation and the trade-off between resolution and phylogenetic coverage.
This listing is for catalog number D6400: Quick-16S NGS Library Prep Kit, 96 Reactions. Instead of endpoint PCR, the kit amplifies the 16S target by real-time qPCR, so product amounts are measured directly and extra library quantification by TapeStation or gel is unnecessary. An enzymatic clean-up between the two PCR steps replaces time-consuming bead purification. The result is a standardized, cost-conscious workflow for 96 reactions that suits labs running microbiome profiling at scale. LIGHT LABS ships genuine Zymo Research products with knowledgeable support from our Englewood, Colorado team.
Key Features
- qPCR-based amplification – Real-time monitoring quantifies products directly during 16S amplification.
- No separate library QC – Removes the need for TapeStation or gel-based quantification.
- Enzymatic clean-up – Replaces lengthy bead-based purification between the two PCR steps.
- Standardized workflow – Brings consistency to 16S rRNA library preparation.
- Scalable format – 96-reaction kit supports large-scale microbiome studies.
Specifications
| Catalog Number | D6400 |
| Brand | Zymo Research |
| Description | Quick-16S NGS Library Prep Kit, 96 Reactions |
| Reactions | 96 reactions |
| Amplification | Real-time (quantitative) PCR |
| Clean-up | Enzymatic clean-up between PCR steps |
| Target | 16S rRNA gene |
| Shipping Conditions | Ships on dry ice |
| Storage | -80C/4C |
Applications
- 16S rRNA amplicon sequencing
- Microbiome composition profiling
- Large-scale microbial ecology studies
- Environmental and clinical research microbiome surveys
