Product Description
The V1-V2 region can offer valuable resolution for certain bacterial groups, and this Zymo Research kit makes sequencing it fast. Hands-on library preparation drops to roughly 30 minutes, giving microbiome labs an automation-friendly path to sequencer-ready V1-V2 amplicon pools. The streamlined workflow also adapts easily to automated liquid handling.
This listing is for catalog number D6434-PS1: Quick-16S Plus NGS Library Prep Kit (V1-V2) with Primer Set 1. Specially designed primers amplify the V1-V2 region of the 16S rRNA gene and add barcodes in one qPCR/PCR reaction. After pooling samples by equal volume, the combined library is cleaned once rather than through many bead purifications, and extra quantification by TapeStation or gel is unnecessary. The kit is supplied with Primer Set 1. Add it to your LIGHT LABS order and keep your bench stocked.
Key Features
- Single-PCR workflow – Combines V1-V2 targeted amplification and barcode addition in one qPCR/PCR step.
- Pool by equal volume – Libraries are pooled without individual normalization.
- One final clean-up – A single clean-up of the pooled library replaces many bead purifications.
- No extra library QC – TapeStation or gel-based quantification is not required.
- About 30 minutes hands-on – Dramatically shortens hands-on library prep time.
- Automation-friendly – Protocol is designed for liquid-handling platforms.
Specifications
| Catalog Number | D6434-PS1 |
| Brand | Zymo Research |
| Description | Quick-16S Plus NGS Library Prep Kit (V1-V2) with Primer Set 1 |
| Target region | 16S rRNA V1-V2 |
| Primer set | Primer Set 1 |
| Hands-on time | About 30 minutes |
| Amplification | Single qPCR/PCR for amplification and barcoding |
| Shipping Conditions | Ships on dry ice |
| Storage | -20C/4C |
| Note | Product contains multiple components shipped separately. (RT and Dry ice) |
Applications
- 16S V1-V2 amplicon sequencing
- Microbiome profiling
- Automated NGS library preparation
- Skin and oral microbiome studies
