Product Description
Covering a longer stretch of the 16S rRNA gene can add taxonomic detail, and this Zymo Research kit makes V1-V3 library preparation quick. Hands-on time falls to about 30 minutes with an automation-friendly protocol that suits high-throughput microbiome sequencing. The protocol is easy to scale for large sample sets and automated platforms.
This listing is for catalog number D6440-PS1: Quick-16S Plus NGS Library Prep Kit (V1-V3) with Primer Set 1. Specially designed primers amplify the V1-V3 region and add barcodes together in a single qPCR/PCR reaction. Samples are pooled by equal volume and the combined library is purified in one clean-up, eliminating repeated bead purifications, while TapeStation or gel quantification is not required. The kit is supplied with Primer Set 1. LIGHT LABS ships genuine Zymo Research products with knowledgeable support from our Englewood, Colorado team.
Key Features
- Single-PCR workflow – Combines V1-V3 targeted amplification and barcode addition in one qPCR/PCR step.
- Pool by equal volume – Libraries are pooled without individual normalization.
- One final clean-up – A single clean-up of the pooled library replaces many bead purifications.
- No extra library QC – TapeStation or gel-based quantification is not required.
- About 30 minutes hands-on – Dramatically shortens hands-on library prep time.
- Automation-friendly – Protocol is designed for liquid-handling platforms.
Specifications
| Catalog Number | D6440-PS1 |
| Brand | Zymo Research |
| Description | Quick-16S Plus NGS Library Prep Kit (V1-V3) with Primer Set 1 |
| Target region | 16S rRNA V1-V3 |
| Primer set | Primer Set 1 |
| Hands-on time | About 30 minutes |
| Amplification | Single qPCR/PCR for amplification and barcoding |
| Shipping Conditions | Ships on dry ice |
| Storage | -20C/4C |
Applications
- 16S V1-V3 amplicon sequencing
- Microbiome composition profiling
- Automated NGS library preparation
- Bacterial taxonomy studies
