Falcon® 352058 | 5mL Round Bottom Polystyrene Test Tube with Dual-Position Snap Cap, Sterile — 25/Pack, 500/Case

Product Description

The Falcon® 352058 is a 5mL round-bottom polystyrene test tube with dual-position snap cap — one of the most widely used and referenced tube formats in laboratory and clinical research worldwide. These tubes come with polyethylene dual-position snap caps, which offer both vented and fully closed positions. RNase/DNase-free. Widely used and referenced in laboratory protocols. Polystyrene tubes are best suited for procedures requiring high optical clarity. Dual-position snap caps, heavier gauge walls, and unique construction provide a secure, positive seal. Rated to 1,400 RCF. Sterile, nonpyrogenicity tested to <0.1 EU/mL. 12 × 75mm. Sold 25 per pack and 500 per case.

Dual-Position Snap Cap — Vented and Fully Closed in One Cap Patented dual snap-cap enables both culturing and storage in the same tube. Leave it loose to allow for aerobic culturing while keeping your sample clean and sterile. The polyethylene dual-position snap cap operates in two distinct positions — fully closed for liquid-tight sample containment during centrifugation, transport, and storage, and partially open (vented) to allow controlled gas exchange during cell culture, biological assays, and incubation workflows requiring CO&sub2; or O&sub2; exchange without exposing the sample to open-air contamination. This dual-position functionality in a single cap eliminates the need to maintain both capped and vented tube formats for different workflow stages.

5mL Format — The Essential Small-Volume Round Bottom Tube The 5mL round-bottom format is the workhorse of flow cytometry, immunology, and hematology laboratories — the ideal volume for antibody staining reactions, cell surface marker panels, red blood cell lysis and washing, density gradient centrifugation of small samples, PBMC staining for flow analysis, and any small-volume suspension cell workflow that benefits from the round-bottom well geometry that concentrates cells to the bottom center of the tube. The 12 × 75mm dimensions fit all standard 5mL tube racks, flow cytometer sample introduction systems, and centrifuge rotor positions designed for this format.

Polystyrene Construction — Exceptional Optical Clarity Polystyrene tubes are best suited for procedures requiring high optical clarity. The clear polystyrene construction of the 352058 provides exceptional optical transparency — critical for visual inspection of cell pellets, sample color changes, media turbidity, and any sample characteristic that must be monitored visually. For flow cytometry applications where researchers frequently inspect tube contents before loading, the optical clarity of the 352058 polystyrene construction is a meaningful practical advantage.

Heavier Gauge Cap Walls — Secure Positive Seal Dual-position snap caps, heavier gauge walls, and unique construction provide a secure, positive seal. The heavier gauge wall construction of the snap cap provides greater mechanical rigidity and resistance to deformation — maintaining positive seal integrity during centrifugation, vortexing, repeated opening and closing cycles, and transport conditions that would cause standard caps to partially open or lose containment.

1,400 RCF Rating — Reliable Centrifugation Performance The 352058 is rated to 1,400 RCF — the standard centrifugal force rating for polystyrene round-bottom tubes used in routine cell biology and clinical centrifugation workflows. This covers the full range of standard applications for this tube format including antibody staining wash steps, cell pelleting for flow cytometry preparation, density gradient centrifugation for serum separation and PBMC isolation, and other routine centrifugation protocols performed within the tube's rated speed range.

Flow Cytometry — The Reference Standard Tube The Falcon 352058 5mL round-bottom tube is the reference standard tube for flow cytometry sample preparation worldwide — specified by catalog number in thousands of published flow cytometry protocols and antibody staining procedures. When an antibody datasheet, flow cytometry panel design guide, or laboratory SOP specifies "5mL Falcon tubes" or "Falcon round-bottom tubes" for staining or acquisition, the 352058 is the correct product. The 12 × 75mm format fits the sample introduction tubes of all major flow cytometers including BD FACSCanto, FACSAria, FACSVerse, Beckman Coulter CytoFLEX, and other standard flow cytometer platforms.

IVF and Reproductive Medicine Applications Round Bottom Tube 5mL intended use is for short storage of culture media, density gradients or cell culture oil (days), collection of follicular fluid (e.g. oocyte collection procedure), processing of semen samples (e.g. swim-up technique) and short storage of processed sperm cells. The Falcon 5mL round-bottom tube is widely used in IVF and andrology laboratories for follicular fluid collection, oocyte and sperm processing, swim-up sperm selection, density gradient centrifugation for sperm preparation, and related reproductive medicine sample handling workflows.

Widely Used and Referenced in Published Protocols Widely used and referenced in laboratory protocols. The Falcon 352058 is cited by catalog number in thousands of peer-reviewed protocols across flow cytometry, immunology, hematology, cell biology, and reproductive medicine — making it the default choice for researchers following established procedures and for core facilities standardizing on published SOPs.

Sterile — Nonpyrogenicity Tested to <0.1 EU/mL Sterile, nonpyrogenicity tested to less than 0.1 EU/mL. Every 352058 is sterile and tested for nonpyrogenicity to <0.1 EU/mL — the endotoxin level standard required for clinical applications, cell culture, and any biological assay where endotoxin contamination could affect cell viability, immune cell activation, or downstream assay results.

RNase-Free and DNase-Free RNase/DNase-free. Certified RNase-free and DNase-free — qualifying the 352058 for RNA isolation, nucleic acid storage, and molecular biology applications where nuclease contamination from the tube material could degrade RNA or DNA samples.

Corning EcoChoice™ — Renewable Energy Manufacturing One or more facilities manufacturing this product is 100% covered by renewable energy through the purchase of unbundled energy attribute certificates (EACs).

Compatible Replacement — Cross-Reference Guide

The Falcon® 352058 is the reference standard for 5mL round-bottom polystyrene tubes. Compatible alternatives include:

• Thermo Fisher Scientific 14-959-1A — Fisherbrand 5mL round bottom polystyrene tube with snap cap, sterile
• Sarstedt 55.1579 — 5mL round bottom polypropylene tube with snap cap, sterile
• VWR® 60818-394 — 5mL round bottom polystyrene tube with cap, sterile
• USA Scientific 1450-2810 — 5mL round bottom tube with snap cap, sterile

Competitor brand names and catalog numbers are referenced for cross-referencing purposes only. Compatibility should be verified for your specific application and protocol.

Complete Falcon® 5mL Round-Bottom Tube Family:

352057 vs. 352058 — 14mL vs. 5mL Round-Bottom Tube:

Key Features

• 5mL round-bottom polystyrene test tube with dual-position snap cap
• Dual-position polyethylene snap cap — vented and fully closed positions
• Patented dual snap-cap enables both culturing and storage in same tube
• Heavier gauge cap walls — secure positive seal
• Clear polystyrene — exceptional optical clarity
• 1,400 RCF centrifugation rating
• 12 × 75mm — fits all standard flow cytometer sample introduction systems
• Reference standard tube for flow cytometry sample preparation
• Widely used and referenced in published laboratory protocols
• IVF and reproductive medicine applications — oocyte collection, swim-up, sperm processing
• Sterile — nonpyrogenicity tested to <0.1 EU/mL
• RNase-free and DNase-free — certified
• No graduations — clear cap color
• Corning EcoChoice™ — renewable energy manufacturing
• 25 tubes per pack / 500 tubes per case

Supported Applications Flow cytometry sample preparation and antibody staining, cell surface marker panels, intracellular staining protocols, PBMC isolation and staining, red blood cell lysis and washing, density gradient centrifugation for small volumes, oocyte collection (IVF), follicular fluid collection and processing, swim-up sperm selection, semen sample processing, small-volume suspension cell culture, cell counting and viability assays, general sample and reagent storage, incubation with gas exchange, and any laboratory application requiring a sterile 5mL round-bottom polystyrene tube with dual-position snap cap.

Technical Specifications