Product Description
LIGHT LABS is proud to offer Mediatech Fetal Bovine Serum (FBS), Regular Grade, USDA Safety Tested (Cat # 35-010-CV) — a high-quality, competitively priced regular-grade FBS for routine mammalian cell culture applications, collected at government-approved harvest facilities in Mexico and Central America and safety tested to USDA standards. The 35-010-CV is the economical workhorse of the Mediatech FBS lineup — providing reliable, consistent cell culture performance for routine maintenance and passaging of established cell lines where the more stringent specifications of Premium grade FBS (35-078-CV) are not required.
Fetal Bovine Serum (FBS) is the most widely used and extensively validated cell culture supplement available — providing a complex, undefined mixture of growth factors, hormones, cell attachment factors, protease inhibitors, vitamins, minerals, lipids, and pH buffering components that collectively satisfy the metabolic requirements of virtually all mammalian cell types in vitro. FBS also protects cells against toxic effects associated with pH change, heavy metal ions, endotoxin exposure, and proteolytic activity — making it an essential component of most standard mammalian cell culture media formulations. The 35-010-CV is sourced exclusively from bovine fetuses collected at USDA-approved harvest facilities — ensuring compliance with the highest North American biosafety standards for FBS collection and processing.
Every lot of the 35-010-CV is true pooled for consistent inter-bottle performance, triple 0.1 µm sterile filtered for maximum sterility assurance, and comprehensively tested — passing sterility, mycoplasma, and 9CFR viral testing requirements on every lot. The 35-010-CV is not heat inactivated — complement activity is preserved in its native state; for heat inactivated USDA FBS, see Cat # 35-011-CV. Storage: -40°C to -10°C (frozen); exceptional 60-month shelf life. Fisher Scientific catalog number MT35010CV. LIGHT LABS is your trusted source for Mediatech Fetal Bovine Serum.
KEY FEATURES
- Regular grade FBS — endotoxin ≤20 EU/mL; hemoglobin <30 mg/dL; the economical choice for routine mammalian cell culture of established cell lines
- USDA Safety Tested — collected at USDA-approved government harvest facilities in Mexico and Central America; meets North American biosafety standards
- Not heat inactivated — complement activity preserved in native state; for heat inactivated format see Cat # 35-011-CV
- True pooled lot — consistent inter-bottle performance; minimizes lot-to-lot variability within a single purchase
- Triple 0.1 µm sterile filtered — highest level of sterility assurance for cell culture applications
- Passes sterility, mycoplasma, and 9CFR viral testing — comprehensive regulatory-grade lot release testing on every lot
- All bovine fetuses collected from USDA-approved harvest facilities — Veterinarian Health Certificate and USDA-APHIS import permit documentation
- Broad cell culture support — provides growth factors, hormones, attachment factors, protease inhibitors, vitamins, minerals, and lipids
- Protects cells against pH toxicity, heavy metal ions, endotoxin, and proteolytic activity
- pH: 7.6 ± 0.8; Osmolality: 315 ± 35 mOsm/kg; Protein: 30–50 g/L
- 60-month shelf life — exceptional frozen stability
- Storage: -40°C to -10°C (frozen); 1 bottle per pack
- Complete Certificate of Analysis available for each production lot
- Fisher Scientific Catalog No. MT35010CV
SPECIFICATIONS
| Attribute | Detail |
|---|---|
| Catalog Number | 35-010-CV |
| Fisher Scientific Catalog No. | MT35010CV |
| Brand | Mediatech |
| Product Name | Fetal Bovine Serum (FBS), Regular, USDA Safety Tested |
| Grade | Regular |
| Origin | USDA Approved — Mexico and Central America |
| Endotoxin Level | ≤20 EU/mL |
| Hemoglobin | <30 mg/dL |
| pH | 7.6 ± 0.8 |
| Osmolality | 315 ± 35 mOsm/kg |
| Protein Content | 30–50 g/L |
| Heat Inactivated | No — NOT heat inactivated |
| Pooling | True pooled lot |
| Sterile Filtration | Triple 0.1 µm sterile filtered |
| Sterility Testing | Yes — each lot |
| Mycoplasma Testing | Yes — each lot |
| Viral Testing | 9CFR viral testing requirements — each lot |
| Color | Amber yellow to reddish |
| Species | Bovine (Bos taurus) |
| Type | Fetal |
| Format | Liquid, sterile, frozen |
| Shelf Life | 60 months |
| Volume | 500 mL |
| Packaging | 1 bottle per pack |
| Storage Temperature | -40°C to -10°C (frozen) |
PREMIUM vs. REGULAR GRADE FBS — KEY DIFFERENCES
| Specification | Regular (35-010-CV) | Premium (35-078-CV) |
|---|---|---|
| Endotoxin | ≤20 EU/mL | ≤5 EU/mL |
| Hemoglobin | <30 mg/dL | <25 mg/dL |
| Origin | USDA Approved (Mexico/Central America) | New Zealand |
| Best For | Routine established cell line culture | Sensitive assays; primary cells; hybridoma |
| Price | More economical | Premium |
USDA FBS — HEAT INACTIVATED vs. NOT HEAT INACTIVATED
| Cat # | Treatment | Grade | Volume |
|---|---|---|---|
| 35-010-CV | None (not heat inactivated) | Regular, USDA | 500 mL |
| 35-011-CV | Heat Inactivated (56°C, 30 min) | Regular, USDA | 500 mL |
WHEN TO CHOOSE HEAT INACTIVATED vs. NOT HEAT INACTIVATED FBS
| Application | Not Heat Inactivated (35-010-CV) | Heat Inactivated (35-011-CV) |
|---|---|---|
| Most routine mammalian cell culture | ✓ Preferred — native state | ✓ Suitable |
| Complement-sensitive assays | ✓ Required | ✗ Complement destroyed |
| Protocols specifically requiring HI FBS | ✗ Not suitable | ✓ Required |
| Hybridoma and antibody production | ✓ Commonly used | ✓ Also used |
APPLICATIONS
- Routine mammalian cell line maintenance and passaging
- Supplementing DMEM, RPMI 1640, MEM, IMDM, DMEM/F-12, and other standard cell culture media at 5–20% v/v
- General cell culture research not requiring premium grade FBS specifications
- High-volume cell culture applications requiring economical regular grade FBS
- Cell line expansion, banking, and cryopreservation
- Any standard mammalian cell culture application requiring USDA safety-tested regular grade FBS
ORDERING INFORMATION — MEDIATECH FETAL BOVINE SERUM
| Cat # | Description | Grade | Origin | Treatment | Volume |
|---|---|---|---|---|---|
| 35-010-CV | FBS, Regular, USDA Safety Tested | Regular | USDA (Mexico/Central America) | None | 500 mL |
| 35-011-CV | FBS, Regular, USDA Safety Tested, Heat Inactivated | Regular | USDA (Mexico/Central America) | Heat Inactivated | 500 mL |
| 35-078-CV | FBS, Premium, New Zealand Origin | Premium | New Zealand | None | 500 mL |
| 35-088-CV | FBS, Premium, New Zealand Origin, Heat Inactivated | Premium | New Zealand | Heat Inactivated | 500 mL |
| 35-079-CV | FBS, Regular, South American Origin | Regular | South American | None | 500 mL |
| 35-077-CV | FBS, Regular, Canadian Origin | Regular | Canada | None | 500 mL |
| 35-017-CV | FBS, Premium, US Origin, Heat Inactivated & Gamma Irradiated | Premium | USA | HI + Gamma | 500 mL |
