MTC Bio OptiWell™ D3096-15S | 96-Well Deep Well Plate, Round U-Bottom, 1.0mL, Sterile — 50/Case (10 Bags of 5)

The MTC Bio OptiWell™ D3096-15S is a 96-well deep well plate with round U-bottom wells and 1.0mL capacity per well — sterile by gamma irradiation, virgin medical-grade polypropylene, black printed alpha-numerics, minimal binding and liquid retention, compatible with all popular automated research platforms and robotic equipment. OptiWell plates come in 24, 48, 96, or 384 format for virtually every research lab, HTS application, and automated/robotic platform. These plates are useful for bacterial cultures, centrifugation (up to 4,000 × g) and general sample storage. Plates are produced and packaged in a Class 100,000 clean room and are ANSI/SLAS 1-2004 compliant. All plates are certified non-pyrogenic and RNase/DNase free. Sterilized by gamma irradiation. Case of 50 plates — 10 bags of 5 plates each.

D3096-15S vs. D3096-15 — Sterile vs. Non-Sterile: The Only Difference The D3096-15S and D3096-15 are identical OptiWell™ 96-well round U-bottom 1.0mL deep well plates — sterility is the sole difference:

The D3096-15S is the correct choice for labs requiring validated sterility in the 1.0mL round U-bottom format — including sterile bacterial culture, sterile cell suspension culture, and any sterile automated liquid handling application requiring this volume and geometry. For non-sterile applications such as sample storage and non-sterile automation, the more economical D3096-15 is the appropriate choice.

Round U-Bottom Wells — Sterile Format for Bacterial Culture and Cell Suspension The round U-bottom well geometry of the D3096-15S promotes efficient resuspension and mixing of bacterial cells, mammalian cells, and beads during shaking incubation and centrifugation — now in a validated sterile format. The rounded well base ensures uniform mixing during shaking culture, supports consistent cell pelleting, and eliminates the residual liquid retention at sharp well corners found in V-bottom formats. For sterile bacterial culture, protein expression in E. coli at 1.0mL scale, and sterile mammalian cell suspension culture in 96-well deep well format, the round U-bottom geometry of the D3096-15S is the preferred sterile format.

D3096-15S vs. D3096-29S — Sterile U-Bottom vs. Sterile Flat-Bottom at Similar Volumes: Both are sterile 96-well round deep well plates — the well bottom geometry and volume are the key differences:

Sterile by Gamma Irradiation — Validated for Bacterial Culture and Sterile Workflows Sterile. Sterilized by gamma irradiation. The D3096-15S is sterilized by gamma irradiation — providing validated, reliable sterility without heat damage or residual chemical concerns. The "S" suffix designates the sterile format — distinguishing it from the non-sterile D3096-15. Gamma irradiation sterilization qualifies the D3096-15S for overnight bacterial liquid culture, protein expression workflows, sterile cell-based assays, and any sterile 96-well deep well application requiring round U-bottom geometry at 1.0mL capacity.

1.0mL Per Well — The Standard Sterile 96-Well Deep Well Culture Volume The 1.0mL per-well capacity of the D3096-15S is the most widely used volume for sterile 96-well deep well bacterial culture workflows — providing sufficient volume for overnight E. coli and other bacterial strain growth with adequate headspace for aeration during shaking incubation. The sterile format ensures that cultures grown in the D3096-15S are free from environmental contamination — critical for reproducible protein expression, genetic screening, and any application where contamination of bacterial cultures would invalidate results.

D3096-15S in the Full OptiWell™ 96-Well Deep Well Family:

Automated Platform Compatibility — ANSI/SLAS 1-2004 Standard For automated research platforms and robotic equipment. The D3096-15S conforms to ANSI/SLAS 1-2004 standard 96-well microplate footprint dimensions — ensuring compatibility with all major automated liquid handling platforms including Hamilton, Tecan, Beckman Coulter, PerkinElmer, Agilent, and other standard HTS robotic systems.

4,000 × g Centrifugation Rating Centrifugation up to 4,000 × g. The robust polypropylene construction of the D3096-15S withstands centrifugal forces up to 4,000 × g — covering all standard plate centrifugation protocols for sterile bacterial cell pelleting, bead collection, and sample concentration at 1.0mL scale.

Virgin Medical-Grade Polypropylene — Minimal Binding and Liquid Retention Minimal binding / liquid retention. Virgin, medical-grade polypropylene. Certified RNase/DNase free and non-pyrogenic.

Black Printed Alpha-Numerics — Instant Well Identification Black printed alpha-numerics. Molded black alpha-numeric well identification (A through H rows, 1 through 12 columns) allows instant identification of specific wells during liquid handling setup and data recording.

Class 100,000 Cleanroom Production — Certified Purity Plates are produced and packaged in a Class 100,000 clean room. All plates are certified non-pyrogenic and RNase/DNase free.

Compatible Replacement — Cross-Reference Guide

The MTC Bio OptiWell™ D3096-15S is a direct compatible replacement for sterile 96-well round U-bottom 1.0mL deep well plates from several major manufacturers:

• Thermo Fisher AB-0661 — Thermo Scientific 96-well round U-bottom deep well plate, 1.0mL, sterile
• Corning® Axygen® P-DW-10-C-S — Axygen 96-well round U-bottom deep well plate, 1.0mL, sterile
• Greiner Bio-One 780271 — 96-well deep well plate, U-bottom, 1.0mL, sterile
• Eppendorf® 0030501306 — Eppendorf 96-well deepwell plate, U-bottom, 1.0mL, sterile

Competitor brand names and catalog numbers are referenced for cross-referencing purposes only. Compatibility should be verified for your specific application and protocol.

Key Features

• MTC Bio OptiWell™ — Catalog # D3096-15S
• 96-well deep well plate — round U-bottom wells
• 1.0mL per-well capacity — standard deep well bacterial culture volume
• Round U-bottom — promotes uniform mixing and resuspension in sterile format
• Sterile — gamma irradiation
• Virgin medical-grade polypropylene — minimal binding and liquid retention
• Black printed alpha-numerics — instant well identification
• ANSI/SLAS 1-2004 compliant — compatible with all standard automated liquid handling platforms
• 4,000 × g centrifugation rating
• Compatible with silicone sealing mats and sealing film
• No lid, no skirt
• Class 100,000 cleanroom production
• Certified RNase-free and DNase-free
• Certified non-pyrogenic
• Non-sterile version: D3096-15
• Case of 50 plates — 10 bags of 5 plates

Supported Applications Sterile 96-well format overnight bacterial liquid culture (E. coli and other organisms), sterile protein expression in bacterial culture at 1.0mL scale, mammalian cell suspension culture in sterile 96-well deep well format, sterile automated liquid handling sample reformatting, sterile bead-based assay preparation, centrifugation workflows up to 4,000 × g, sterile reagent preparation, sterile compound dilution series, and any 96-well application requiring sterile round U-bottom deep well format with 1.0mL per-well capacity and full automated platform compatibility.

Technical Specifications