Product Description
The MTC Bio OptiWell™ D3096-21S is a 96-well deep well plate with round U-bottom wells and 1.1mL capacity per well — sterile by gamma irradiation, virgin medical-grade polypropylene, black printed alpha-numerics, minimal binding and liquid retention, compatible with all popular automated research platforms and robotic equipment. OptiWell plates come in 24, 48, 96, or 384 format for virtually every research lab, HTS application, and automated/robotic platform. These plates are useful for bacterial cultures, centrifugation (up to 4,000 × g) and general sample storage. Plates are produced and packaged in a Class 100,000 clean room and are ANSI/SLAS 1-2004 compliant. All plates are certified non-pyrogenic and RNase/DNase free. Sterilized by gamma irradiation. Case of 50 plates — 10 bags of 5 plates each.
D3096-21S vs. D3096-21 — Sterile vs. Non-Sterile: The Only Difference The D3096-21S and D3096-21 are identical OptiWell™ 96-well round U-bottom 1.1mL deep well plates — sterility is the sole difference:
| D3096-21S | D3096-21 | |
|---|---|---|
| Sterility | ✅ Sterile — gamma irradiation | ❌ Non-sterile |
| Best For | Bacterial culture, sterile protein expression, sterile cell-based workflows | General sample storage, non-sterile automation, compound management |
| Volume | 1.1mL | 1.1mL |
| Well Bottom | U-bottom | U-bottom |
| Well Shape | Round | Round |
| Cost | Higher | Lower |
| Case Qty | 50 | 50 |
The D3096-21S is the correct choice for labs requiring validated sterility in the 1.1mL round U-bottom format — including sterile bacterial culture, sterile protein expression in E. coli, and any sterile automated liquid handling application requiring this specific volume and geometry. For non-sterile applications, the more economical D3096-21 is the appropriate choice.
Round U-Bottom Wells — Sterile Format for Bacterial Culture and Cell Suspension The round U-bottom well geometry of the D3096-21S promotes efficient resuspension and mixing of bacterial cells, mammalian cells, and beads during shaking incubation and centrifugation — now in a validated sterile format. The rounded well base ensures uniform mixing during shaking culture, supports consistent cell pelleting, and eliminates residual liquid retention at sharp well corners. For sterile bacterial culture, sterile protein expression in E. coli at 1.1mL scale, and sterile cell suspension workflows where the 1.0mL format is slightly insufficient, the D3096-21S provides the precisely right sterile volume.
1.1mL Per Well — The Sterile Mid-Volume U-Bottom Format The 1.1mL per-well capacity of the D3096-21S fills the precise volume niche between the sterile 1.0mL round U-bottom (D3096-15S) and the sterile 1.2mL flat-bottom (D3096-29S) — providing sterile working capacity at the exact 1.1mL volume for protocols optimized at this scale. For sterile bacterial culture workflows where 1.0mL is slightly insufficient for robust overnight growth but 2.2mL provides excessive headspace, the D3096-21S delivers the optimal sterile format.
D3096-21S vs. D3096-15S — Sterile 1.1mL vs. 1.0mL Round U-Bottom:
| D3096-21S | D3096-15S | |
|---|---|---|
| Volume | 1.1mL | 1.0mL |
| Well Shape | Round | Round |
| Well Bottom | U-bottom | U-bottom |
| Sterile | Yes | Yes |
| Case Qty | 50 | 50 |
| Best For | Protocols requiring 1.1mL sterile culture volume | Standard 1.0mL sterile bacterial culture |
D3096-21S vs. D3096-29S — Sterile U-Bottom vs. Sterile Flat-Bottom at Similar Volumes:
| D3096-21S | D3096-29S | |
|---|---|---|
| Volume | 1.1mL | 1.2mL |
| Well Bottom | U-bottom | Flat |
| Well Shape | Round | Round |
| Sterile | Yes | Yes |
| Case Qty | 50 | 50 |
| Best For | Bacterial culture, mixing, resuspension — sterile | OD reading, absorbance, optical detection — sterile |
D3096-21S in the Full OptiWell™ 96-Well Deep Well Family:
| Cat. # | Wells | Bottom | Volume | Sterile | Case Qty |
|---|---|---|---|---|---|
| D3096-07 | 96 Round | V-bottom | 0.45mL | No | 100 |
| D3096-07S | 96 Round | V-bottom | 0.45mL | Yes | 100 |
| D3096-09 | 96 Square | V-bottom | 0.5mL | No | 100 |
| D3096-09S | 96 Square | V-bottom | 0.5mL | Yes | 100 |
| D3096-15 | 96 Round | U-bottom | 1.0mL | No | 50 |
| D3096-15S | 96 Round | U-bottom | 1.0mL | Yes | 50 |
| D3096-19 | 96 Square | V-bottom | 1.0mL | No | 100 |
| D3096-19S | 96 Square | V-bottom | 1.0mL | Yes | 100 |
| D3096-21 | 96 Round | U-bottom | 1.1mL | No | 50 |
| D3096-21S | 96 Round | U-bottom | 1.1mL | Yes | 50 |
| D3096-29 | 96 Round | Flat bottom | 1.2mL | No | 50 |
| D3096-29S | 96 Round | Flat bottom | 1.2mL | Yes | 50 |
| D3096-41 | 96 Square | U-bottom Standard | 2.2mL | No | 50 |
| D3096-41S | 96 Square | U-bottom Standard | 2.2mL | Yes | 50 |
| D3096-43 | 96 Square | U-bottom H-style | 2.2mL | No | 50 |
| D3096-43S | 96 Square | U-bottom H-style | 2.2mL | Yes | 50 |
Sterile by Gamma Irradiation — Validated for Bacterial Culture and Sterile Workflows Sterile. Sterilized by gamma irradiation. The D3096-21S is sterilized by gamma irradiation — providing validated, reliable sterility without heat damage or residual chemical concerns. The "S" suffix designates the sterile format. Gamma irradiation sterilization qualifies the D3096-21S for overnight bacterial liquid culture at 1.1mL scale, sterile protein expression, sterile cell-based assays, and any sterile 96-well deep well application requiring round U-bottom geometry at 1.1mL capacity.
Automated Platform Compatibility — ANSI/SLAS 1-2004 Standard For automated research platforms and robotic equipment. The D3096-21S conforms to ANSI/SLAS 1-2004 standard 96-well microplate footprint dimensions — ensuring compatibility with all major automated liquid handling platforms including Hamilton, Tecan, Beckman Coulter, PerkinElmer, Agilent, and other standard HTS robotic systems.
4,000 × g Centrifugation Rating Centrifugation up to 4,000 × g — covering all standard plate centrifugation protocols for sterile bacterial cell pelleting, bead collection, and sample concentration at 1.1mL scale.
Virgin Medical-Grade Polypropylene — Minimal Binding and Liquid Retention Minimal binding / liquid retention. Virgin, medical-grade polypropylene. Certified RNase/DNase free and non-pyrogenic.
Black Printed Alpha-Numerics — Instant Well Identification Black printed alpha-numerics. Molded black alpha-numeric well identification (A through H rows, 1 through 12 columns).
Class 100,000 Cleanroom Production — Certified Purity Plates are produced and packaged in a Class 100,000 clean room. Certified non-pyrogenic and RNase/DNase free.
Compatible Replacement — Cross-Reference Guide
The MTC Bio OptiWell™ D3096-21S is a direct compatible replacement for sterile 96-well round U-bottom 1.1mL deep well plates from several major manufacturers:
- Thermo Fisher AB-0661 — Thermo Scientific 96-well round U-bottom deep well plate, 1.1mL, sterile
- Greiner Bio-One 780271 — 96-well deep well plate, round U-bottom, 1.1mL, sterile
- Eppendorf® 0030501306 — Eppendorf 96-well deepwell plate, round U-bottom, 1.1mL, sterile
Competitor brand names and catalog numbers are referenced for cross-referencing purposes only. Compatibility should be verified for your specific application and protocol.
Key Features
- MTC Bio OptiWell™ — Catalog # D3096-21S
- 96-well deep well plate — round U-bottom wells
- 1.1mL per-well capacity — unique sterile volume between 1.0mL and 1.2mL formats
- Round U-bottom — promotes uniform mixing and resuspension in sterile format
- Sterile — gamma irradiation
- Virgin medical-grade polypropylene — minimal binding and liquid retention
- Black printed alpha-numerics — instant well identification
- ANSI/SLAS 1-2004 compliant — compatible with all standard automated liquid handling platforms
- 4,000 × g centrifugation rating
- Compatible with silicone sealing mats and sealing film
- No lid, no skirt
- Class 100,000 cleanroom production
- Certified RNase-free and DNase-free
- Certified non-pyrogenic
- Non-sterile version: D3096-21
- Case of 50 plates — 10 bags of 5 plates
Supported Applications Sterile 96-well format bacterial liquid culture at 1.1mL scale, sterile protein expression in E. coli at 1.1mL culture volume, sterile mammalian cell suspension culture in 96-well deep well format, sterile automated liquid handling sample processing, sterile compound dilution and preparation at 1.1mL scale, centrifugation workflows up to 4,000 × g, and any 96-well application requiring sterile round U-bottom deep well format with 1.1mL per-well capacity and full automated platform compatibility.
Technical Specifications
| Specification | Value |
|---|---|
| SKU / Catalog # | D3096-21S |
| Manufacturer | MTC Bio |
| Product Line | OptiWell™ |
| Well Format | 96-well |
| Well Shape | Round |
| Well Bottom | U-bottom (round) |
| Well Volume / Capacity | 1.1mL per well |
| Material | Virgin medical-grade polypropylene |
| Color | Clear |
| Alpha-Numerics | Yes — black printed |
| Lid | No |
| Skirt | No |
| Centrifugation Rating | 4,000 × g |
| Binding | Minimal — low binding and liquid retention |
| Sealing Compatibility | Silicone sealing mats and sealing film |
| Sterility | Sterile — gamma irradiation |
| Non-Sterile Version | D3096-21 |
| Footprint | ANSI/SLAS 1-2004 compliant |
| Production Environment | Class 100,000 cleanroom |
| RNase-Free | Yes — certified |
| DNase-Free | Yes — certified |
| Non-Pyrogenic | Yes — certified |
| Case Quantity | 50 plates (10 bags × 5 plates) |