Product Description
The MTC Bio OptiWell™ D3096-21 is a 96-well deep well plate with round U-bottom wells and 1.1mL capacity per well — non-sterile, virgin medical-grade polypropylene, black printed alpha-numerics, minimal binding and liquid retention, compatible with all popular automated research platforms and robotic equipment. OptiWell plates come in 24, 48, 96, or 384 format for virtually every research lab, HTS application, and automated/robotic platform. These plates are useful for bacterial cultures, centrifugation (up to 4,000 × g) and general sample storage. Plates are produced and packaged in a Class 100,000 clean room and are ANSI/SLAS 1-2004 compliant. All plates are certified non-pyrogenic and RNase/DNase free. Case of 50 plates — 10 bags of 5 plates each.
Round U-Bottom Wells — Optimal for Bacterial Culture, Mixing, and Cell Suspension The round U-bottom well geometry of the D3096-21 promotes efficient resuspension and mixing of bacterial cells, mammalian cells, and beads during shaking incubation and centrifugation. The rounded well base ensures uniform mixing during shaking culture, supports consistent cell pelleting, and eliminates the residual liquid retention at sharp well corners found in V-bottom formats. For bacterial culture, protein expression, and cell suspension workflows where even mixing and complete resuspension are more important than maximum sample recovery, the round U-bottom geometry of the D3096-21 is the preferred format.
1.1mL Per Well — Between Standard 1.0mL and 1.2mL Formats The 1.1mL per-well capacity of the D3096-21 occupies a precise volume niche between the standard 1.0mL round U-bottom format (D3096-15) and the 1.2mL flat-bottom format (D3096-29) — providing a meaningful 10% additional volume over the D3096-15 for bacterial culture protocols that require slightly more medium volume for robust overnight growth without stepping up to the larger formats. For labs whose existing bacterial culture protocols are specifically optimized for 1.0–1.1mL culture volumes, the D3096-21 provides the exact volume match without excess headspace.
D3096-21 vs. D3096-15 — 1.1mL vs. 1.0mL Round U-Bottom Non-Sterile:
| D3096-21 | D3096-15 | |
|---|---|---|
| Volume | 1.1mL | 1.0mL |
| Well Shape | Round | Round |
| Well Bottom | U-bottom | U-bottom |
| Sterile | No | No |
| Case Qty | 50 | 50 |
| Best For | Protocols requiring 1.1mL culture volume | Standard 1.0mL bacterial culture and mixing |
D3096-21 vs. D3096-29 — Round U-Bottom vs. Round Flat-Bottom at Similar Volumes:
| D3096-21 | D3096-29 | |
|---|---|---|
| Volume | 1.1mL | 1.2mL |
| Well Bottom | U-bottom | Flat |
| Well Shape | Round | Round |
| Sterile | No | No |
| Best For | Bacterial culture, mixing, resuspension | OD reading, absorbance, optical detection |
| Case Qty | 50 | 50 |
D3096-21 in the Full OptiWell™ 96-Well Deep Well Family:
| Cat. # | Wells | Bottom | Volume | Sterile | Case Qty |
|---|---|---|---|---|---|
| D3096-07 | 96 Round | V-bottom | 0.45mL | No | 100 |
| D3096-07S | 96 Round | V-bottom | 0.45mL | Yes | 100 |
| D3096-09 | 96 Square | V-bottom | 0.5mL | No | 100 |
| D3096-09S | 96 Square | V-bottom | 0.5mL | Yes | 100 |
| D3096-15 | 96 Round | U-bottom | 1.0mL | No | 50 |
| D3096-15S | 96 Round | U-bottom | 1.0mL | Yes | 50 |
| D3096-19 | 96 Square | V-bottom | 1.0mL | No | 100 |
| D3096-19S | 96 Square | V-bottom | 1.0mL | Yes | 100 |
| D3096-21 | 96 Round | U-bottom | 1.1mL | No | 50 |
| D3096-29 | 96 Round | Flat bottom | 1.2mL | No | 50 |
| D3096-29S | 96 Round | Flat bottom | 1.2mL | Yes | 50 |
| D3096-41 | 96 Square | U-bottom Standard | 2.2mL | No | 50 |
| D3096-41S | 96 Square | U-bottom Standard | 2.2mL | Yes | 50 |
| D3096-43 | 96 Square | U-bottom H-style | 2.2mL | No | 50 |
| D3096-43S | 96 Square | U-bottom H-style | 2.2mL | Yes | 50 |
Automated Platform Compatibility — ANSI/SLAS 1-2004 Standard For automated research platforms and robotic equipment. The D3096-21 conforms to ANSI/SLAS 1-2004 standard 96-well microplate footprint dimensions — ensuring compatibility with all major automated liquid handling platforms including Hamilton, Tecan, Beckman Coulter, PerkinElmer, Agilent, and other standard HTS robotic systems.
4,000 × g Centrifugation Rating Centrifugation up to 4,000 × g. The robust polypropylene construction of the D3096-21 withstands centrifugal forces up to 4,000 × g — covering all standard plate centrifugation protocols for bacterial cell pelleting, sample concentration, and bead collection at 1.1mL scale.
Virgin Medical-Grade Polypropylene — Minimal Binding and Liquid Retention Minimal binding / liquid retention. Virgin, medical-grade polypropylene. Manufactured from virgin medical-grade polypropylene — free from recycled resin contaminants and extractables. Certified RNase/DNase free and non-pyrogenic.
Black Printed Alpha-Numerics — Instant Well Identification Black printed alpha-numerics. Molded black alpha-numeric well identification (A through H rows, 1 through 12 columns).
Class 100,000 Cleanroom Production — Certified Purity Plates are produced and packaged in a Class 100,000 clean room. Certified non-pyrogenic and RNase/DNase free.
Compatible Replacement — Cross-Reference Guide
The MTC Bio OptiWell™ D3096-21 is a direct compatible replacement for non-sterile 96-well round U-bottom 1.1mL deep well plates from several major manufacturers:
- Thermo Fisher AB-0661 — Thermo Scientific 96-well round U-bottom deep well plate, 1.1mL, non-sterile
- Corning® Axygen® P-DW-10-C — Axygen 96-well round U-bottom deep well plate, 1.1mL, non-sterile
- Greiner Bio-One 780271 — 96-well deep well plate, round U-bottom, 1.1mL, non-sterile
- Eppendorf® 0030501306 — Eppendorf 96-well deepwell plate, round U-bottom, 1.1mL, non-sterile
Competitor brand names and catalog numbers are referenced for cross-referencing purposes only. Compatibility should be verified for your specific application and protocol.
Key Features
- MTC Bio OptiWell™ — Catalog # D3096-21
- 96-well deep well plate — round U-bottom wells
- 1.1mL per-well capacity — unique volume between 1.0mL and 1.2mL formats
- Round U-bottom — promotes uniform mixing and resuspension for bacterial culture
- Non-sterile
- Virgin medical-grade polypropylene — minimal binding and liquid retention
- Black printed alpha-numerics — instant well identification
- ANSI/SLAS 1-2004 compliant — compatible with all standard automated liquid handling platforms
- 4,000 × g centrifugation rating
- Compatible with silicone sealing mats and sealing film
- No lid, no skirt
- Autoclavable — can be sterilized before use if required
- Class 100,000 cleanroom production
- Certified RNase-free and DNase-free
- Certified non-pyrogenic
- Sigma-Aldrich catalog # MTCD309621
- Case of 50 plates — 10 bags of 5 plates
Supported Applications 96-well format bacterial liquid culture at 1.1mL scale, protein expression in E. coli at 1.1mL culture volume, cell suspension culture in 96-well deep well format, automated liquid handling sample reformatting, compound dilution and preparation at 1.1mL scale, bead-based assay preparation, centrifugation workflows up to 4,000 × g, general sample storage and archiving, and any 96-well application requiring round U-bottom deep well format with 1.1mL per-well capacity and full automated platform compatibility.
Technical Specifications
| Specification | Value |
|---|---|
| SKU / Catalog # | D3096-21 |
| Sigma-Aldrich Catalog # | MTCD309621 |
| Manufacturer | MTC Bio |
| Product Line | OptiWell™ |
| Well Format | 96-well |
| Well Shape | Round |
| Well Bottom | U-bottom (round) |
| Well Volume / Capacity | 1.1mL per well |
| Material | Virgin medical-grade polypropylene |
| Color | Clear |
| Alpha-Numerics | Yes — black printed |
| Lid | No |
| Skirt | No |
| Centrifugation Rating | 4,000 × g |
| Binding | Minimal — low binding and liquid retention |
| Sealing Compatibility | Silicone sealing mats and sealing film |
| Sterility | Non-sterile |
| Autoclavable | Yes |
| Footprint | ANSI/SLAS 1-2004 compliant |
| Production Environment | Class 100,000 cleanroom |
| RNase-Free | Yes — certified |
| DNase-Free | Yes — certified |
| Non-Pyrogenic | Yes — certified |
| Case Quantity | 50 plates (10 bags × 5 plates) |