MTC Bio OptiWell™ D3096-41S | 96-Well Deep Well Plate, Square U-Bottom Standard, 2.2mL, Sterile — 50/Case (10 Bags of 5)

The MTC Bio OptiWell™ D3096-41S is a 96-well deep well plate with square U-bottom standard wells and 2.2mL capacity per well — sterile by gamma irradiation, virgin medical-grade polypropylene, black printed alpha-numerics, minimal binding and liquid retention, compatible with all popular automated research platforms and robotic equipment. OptiWell plates come in 24, 48, 96, or 384 format for virtually every research lab, HTS application, and automated/robotic platform. These plates are useful for bacterial cultures, centrifugation (up to 4,000 × g) and general sample storage. Plates are produced and packaged in a Class 100,000 clean room and are ANSI/SLAS 1-2004 compliant. All plates are certified non-pyrogenic and RNase/DNase free. Sterilized by gamma irradiation. Case of 50 plates — 10 bags of 5 plates each.

Square U-Bottom Standard Wells — Maximum Volume in the General-Purpose Sterile Deep Well Format The square U-bottom standard well geometry of the D3096-41S is the most widely used format for general-purpose 96-well deep well plate applications — providing the maximum 2.2mL working volume per well within the ANSI/SLAS standard 96-well footprint while the standard square well cross-section ensures broad compatibility across all automated liquid handling platforms, robotic sample processors, and manual multichannel pipetting workflows. The "standard" designation distinguishes this plate from the H-style geometry of the D3096-43S — the standard format features straight square well walls with a U-bottom rather than the specific curvature profile of the H-style format designed for magnetic tip comb systems. For labs requiring the 2.2mL sterile deep well format for bacterial culture, general automation, and sample processing workflows that do not specifically require the H-style geometry, the D3096-41S is the correct and more universally compatible format.

D3096-41S vs. D3096-43S — Standard vs. H-Style at 2.2mL Sterile: The Key Distinction Both the D3096-41S and D3096-43S are sterile 96-well square U-bottom 2.2mL deep well plates — the well geometry designation is the critical differentiator:

D3096-41S vs. D3096-41 — Sterile vs. Non-Sterile: The Only Other Difference

2.2mL Per Well — Maximum Volume for 96-Well Deep Well Applications The 2.2mL per-well capacity of the D3096-41S is the largest standard volume in the 96-well deep well format — providing the maximum working volume for overnight bacterial liquid culture, large-volume sample processing, high-throughput protein expression, and any 96-well workflow requiring maximum well volume. For bacterial culture workflows in particular, the 2.2mL capacity accommodates overnight growth of E. coli and other bacterial strains with adequate headspace for aeration during shaking incubation at standard deep well culture volumes of 0.5–1.5mL.

Sterile by Gamma Irradiation — ISO 13485 Validated Sterile. Sterilized by gamma irradiation. The D3096-41S is sterilized by gamma irradiation in accordance with ISO 13485 — providing validated, reliable sterility without heat damage or residual chemical concerns. The "S" suffix designates the sterile format — distinguishing it from the non-sterile D3096-41. Gamma irradiation sterilization qualifies the D3096-41S for bacterial culture, mammalian cell culture, sterile nucleic acid processing, and any application requiring validated sterile plasticware.

D3096-41S in the OptiWell™ 96-Well Deep Well Family:

Automated Platform Compatibility — ANSI/SLAS 1-2004 Standard For automated research platforms and robotic equipment. The D3096-41S conforms to ANSI/SLAS 1-2004 standard 96-well microplate footprint dimensions — ensuring compatibility with all major automated liquid handling platforms including Hamilton, Tecan, Beckman Coulter, PerkinElmer, Agilent, and other standard HTS robotic systems.

4,000 × g Centrifugation Rating Centrifugation up to 4,000 × g. The robust polypropylene construction of the D3096-41S withstands centrifugal forces up to 4,000 × g — covering all standard plate centrifugation protocols including bacterial cell pelleting, bead collection, and sample concentration.

Virgin Medical-Grade Polypropylene — Minimal Binding and Liquid Retention Minimal binding / liquid retention. Virgin, medical-grade polypropylene. Manufactured from virgin medical-grade polypropylene — free from recycled resin contaminants, processing aids, and extractables. Certified RNase/DNase free and non-pyrogenic.

Black Printed Alpha-Numerics — Instant Well Identification Black printed alpha-numerics. Molded black alpha-numeric well identification (A through H rows, 1 through 12 columns) allows instant identification of specific wells during liquid handling setup, plate reading, and data recording.

Compatible Replacement — Cross-Reference Guide

The MTC Bio OptiWell™ D3096-41S is a direct compatible replacement for sterile 96-well square U-bottom standard 2.2mL deep well plates from several major manufacturers:

• Greiner Bio-One 780270 — 96-well deep well plate, square U-bottom, 2.2mL, sterile
• Eppendorf® 0030501328 — Eppendorf 96-well deepwell plate, square U-bottom, 2.2mL, sterile
• Thermo Fisher AB-0932 — Thermo Scientific 96-well square U-bottom deep well plate, 2.2mL, sterile

Competitor brand names and catalog numbers are referenced for cross-referencing purposes only. Compatibility should be verified for your specific application and protocol.

Key Features

• MTC Bio OptiWell™ — Catalog # D3096-41S
• 96-well deep well plate — square U-bottom standard wells
• 2.2mL per-well capacity — maximum volume in 96-well deep well format
• Standard square U-bottom — broadest automated platform compatibility
• Sterile — gamma irradiation, ISO 13485
• Virgin medical-grade polypropylene — minimal binding and liquid retention
• Black printed alpha-numerics — instant well identification
• ANSI/SLAS 1-2004 compliant — compatible with all standard automated liquid handling platforms
• 4,000 × g centrifugation rating
• Compatible with silicone sealing mats and sealing film
• No lid, no skirt
• Class 100,000 cleanroom production
• Certified RNase-free and DNase-free
• Certified non-pyrogenic
• Sigma-Aldrich catalog # MTCD309641S
• Case of 50 plates — 10 bags of 5 plates

Supported Applications 96-well format overnight bacterial liquid culture (E. coli and other organisms), mammalian cell culture in 96-well deep well format, high-throughput protein expression in bacterial culture, automated liquid handling sample reformatting requiring sterile plates, compound dilution and preparation at 2.2mL scale, bead-based assay preparation, centrifugation workflows up to 4,000 × g, sterile nucleic acid sample processing, sterile sample storage and archiving, and any 96-well application requiring standard square U-bottom deep well format at 2.2mL capacity with validated sterility and full automated platform compatibility.

Technical Specifications