MTC Bio OptiWell™ D3096-43 | 96-Well Deep Well Plate, Square U-Bottom H-Style, 2.2mL, Non-Sterile — 50/Case (10 Bags of 5)

The MTC Bio OptiWell™ D3096-43 is a 96-well deep well plate with square U-bottom H-style wells and 2.2mL capacity per well — non-sterile, virgin medical-grade polypropylene, black printed alpha-numerics, minimal binding and liquid retention, compatible with all popular automated research platforms and robotic equipment including KingFisher™ and Accuris IsoPure™ magnetic bead platforms. OptiWell plates come in 24, 48, 96, or 384 format for virtually every research lab, HTS application, and automated/robotic platform. These plates are useful for bacterial cultures, centrifugation (up to 4,000 × g) and general sample storage. Plates are produced and packaged in a Class 100,000 clean room and are ANSI/SLAS 1-2004 compliant. All plates are certified non-pyrogenic and RNase/DNase free. Case of 50 plates — 10 bags of 5 plates each. The sterile version is available as D3096-43S.

D3096-43 vs. D3096-43S — Non-Sterile vs. Sterile: Choosing the Right Format The D3096-43 and D3096-43S are identical OptiWell™ 96-well square U-bottom H-style 2.2mL deep well plates — sterility is the sole difference:

The D3096-43 non-sterile format is the correct choice for labs running non-sterile magnetic bead workflows — including KingFisher™ and Accuris IsoPure™ automated nucleic acid extraction workflows where sterile plates are not required, non-sterile compound management, and any 2.2mL H-style application where plate contents do not require a sterile vessel. For bacterial culture and sterile nucleic acid extraction workflows where sterility is required, the D3096-43S is the appropriate choice.

D3096-43 vs. D3096-41 — H-Style vs. Standard at 2.2mL Non-Sterile: Both are non-sterile 96-well square U-bottom 2.2mL deep well plates — the well geometry designation is the critical differentiator:

Square U-Bottom H-Style Wells — The Non-Sterile Standard for Magnetic Bead Automation The square U-bottom H-style well geometry of the D3096-43 is the recognized format for 96-well deep well plates used in magnetic bead-based nucleic acid extraction workflows on automated platforms — now in a non-sterile format for applications where sterility is not required. The H-style geometry is specifically optimized for compatibility with KingFisher™ Flex and Duo Prime magnetic particle processors, Accuris IsoPure™ magnetic bead systems, and other automated magnetic bead DNA/RNA extraction platforms — providing the correct well geometry for magnetic tip comb systems at the 2.2mL lysis and binding buffer scale used in automated extraction protocols.

2.2mL Per Well — Maximum Volume for Non-Sterile Magnetic Bead and Deep Well Workflows The 2.2mL per-well capacity provides the maximum working volume for non-sterile automated magnetic bead extraction workflows — accommodating the full volume of lysis buffer and binding buffer required for complete nucleic acid extraction protocols without well overflow during bead mixing steps. As the non-sterile format, the D3096-43 provides this maximum volume at the lowest per-plate cost in the H-style 2.2mL family — making it the most economical choice for high-volume non-sterile magnetic bead extraction workflows where plates are consumed in large quantities.

KingFisher™ and Accuris IsoPure™ Compatibility — Non-Sterile Format The D3096-43 carries the same compatibility with KingFisher™ and Accuris IsoPure™ automated magnetic bead extraction platforms as the sterile D3096-43S — in a non-sterile format for labs that do not require sterile plates for their automated extraction workflow. Many non-sterile nucleic acid extraction workflows — including DNA extraction from environmental samples, plant material, food, and other non-clinical sources — do not require sterile plates, making the D3096-43 the more economical choice for these applications.

D3096-43 in the Full OptiWell™ 96-Well Deep Well Family:

Automated Platform Compatibility — ANSI/SLAS 1-2004 Standard For automated research platforms and robotic equipment. The D3096-43 conforms to ANSI/SLAS 1-2004 standard 96-well microplate footprint dimensions — ensuring compatibility with all major automated liquid handling platforms including Hamilton, Tecan, Beckman Coulter, PerkinElmer, Agilent, KingFisher™, Accuris IsoPure™, and other standard HTS robotic systems.

4,000 × g Centrifugation Rating Centrifugation up to 4,000 × g. The robust polypropylene construction of the D3096-43 withstands centrifugal forces up to 4,000 × g — covering all standard plate centrifugation protocols for bead collection, sample concentration, and processing at 2.2mL scale.

Virgin Medical-Grade Polypropylene — Minimal Binding and Liquid Retention Minimal binding / liquid retention. Virgin, medical-grade polypropylene. Manufactured from virgin medical-grade polypropylene — free from recycled resin contaminants and extractables. Certified RNase/DNase free and non-pyrogenic — the non-sterile designation refers only to the absence of gamma irradiation sterilization, not to the purity of the polypropylene resin.

Black Printed Alpha-Numerics — Instant Well Identification Black printed alpha-numerics. Molded black alpha-numeric well identification (A through H rows, 1 through 12 columns) allows instant identification of specific wells during liquid handling setup and data recording.

Class 100,000 Cleanroom Production — Certified Purity Plates are produced and packaged in a Class 100,000 clean room. Certified non-pyrogenic and RNase/DNase free — maintaining molecular biology grade purity standards despite the non-sterile designation.

Compatible Replacement — Cross-Reference Guide

The MTC Bio OptiWell™ D3096-43 is a direct compatible replacement for non-sterile 96-well square U-bottom H-style 2.2mL deep well plates from several major manufacturers:

• Thermo Fisher 95040460 — KingFisher™ 96 deep well plate, square U-bottom, 2.2mL, non-sterile
• Corning® Axygen® P-DW-20-C — Axygen 96-well square U-bottom deep well plate, 2.2mL, non-sterile
• Greiner Bio-One 780270 — 96-well deep well plate, square U-bottom, 2.2mL, non-sterile
• Eppendorf® 0030501328 — Eppendorf 96-well deepwell plate, square U-bottom, 2.2mL, non-sterile

Competitor brand names and catalog numbers are referenced for cross-referencing purposes only. Compatibility should be verified for your specific application and protocol.

Key Features

• MTC Bio OptiWell™ — Catalog # D3096-43
• 96-well deep well plate — square U-bottom H-style wells
• 2.2mL per-well capacity — maximum volume in 96-well deep well format
• Square U-bottom H-style — compatible with KingFisher™ and Accuris IsoPure™ magnetic bead platforms
• Non-sterile — lowest per-plate cost in the H-style 2.2mL family
• Virgin medical-grade polypropylene — minimal binding and liquid retention
• Black printed alpha-numerics — instant well identification
• ANSI/SLAS 1-2004 compliant — compatible with all standard automated liquid handling platforms
• 4,000 × g centrifugation rating
• Compatible with silicone sealing mats and sealing film
• No lid, no skirt
• Autoclavable — can be sterilized before use if required
• Class 100,000 cleanroom production
• Certified RNase-free and DNase-free
• Certified non-pyrogenic
• Sterile version: D3096-43S
• Case of 50 plates — 10 bags of 5 plates

Supported Applications Non-sterile automated magnetic bead nucleic acid extraction on KingFisher™ Flex and Duo Prime platforms, non-sterile Accuris IsoPure™ magnetic bead extraction, environmental DNA/RNA extraction in automated format, plant and food sample nucleic acid extraction, non-sterile automated liquid handling at 2.2mL scale, compound dilution and preparation, general sample storage, centrifugation workflows up to 4,000 × g, and any 96-well application requiring square U-bottom H-style deep well format at 2.2mL capacity without a sterility requirement.

Technical Specifications