Product Description
The MTC Bio OptiWell™ D3096-25 is a 96-well deep well plate with square U-bottom wells and 1.2mL capacity per well — non-sterile, virgin medical-grade polypropylene, black printed alpha-numerics, minimal binding and liquid retention, compatible with all popular automated research platforms and robotic equipment. OptiWell plates come in 24, 48, 96, or 384 format for virtually every research lab, HTS application, and automated/robotic platform. These plates are useful for bacterial cultures, centrifugation (up to 4,000 × g) and general sample storage. Plates are produced and packaged in a Class 100,000 clean room and are ANSI/SLAS 1-2004 compliant. All plates are certified non-pyrogenic and RNase/DNase free. Case of 50 plates — 10 bags of 5 plates each.
Square U-Bottom Wells — Maximum Volume Efficiency with Uniform Mixing at 1.2mL The square U-bottom well geometry of the D3096-25 combines the space-maximizing capacity of a square well cross-section with the rounded base of a U-bottom — delivering the maximum volume capacity within the ANSI/SLAS standard 96-well footprint while the rounded well base promotes efficient resuspension and uniform mixing of cells, beads, and precipitates during shaking incubation and centrifugation. For non-sterile bacterial culture, mixing-dependent bead assays, and general sample storage where the square well's volume advantage over round wells is important, the D3096-25 square U-bottom format provides the optimal combination of volume efficiency and mixing performance at 1.2mL scale.
1.2mL Per Well — Square U-Bottom at Mid-Deep Well Volume The 1.2mL per-well capacity of the D3096-25 provides a meaningful volume step up from the 1.0–1.1mL round formats while the square well cross-section delivers more working volume than a round well of equivalent depth — making the D3096-25 the highest-volume non-sterile mid-range deep well option in the LIGHT LABS OptiWell™ lineup below the 2.2–2.3mL large-format plates. For non-sterile bacterial culture protocols that require the combined advantages of square well volume and U-bottom mixing geometry at 1.2mL scale, the D3096-25 is the specifically correct format.
D3096-25 vs. D3096-29 — Square U-Bottom vs. Round Flat-Bottom at 1.2mL Non-Sterile: Both plates are non-sterile 96-well 1.2mL deep well plates — the well shape and bottom geometry are the critical differentiators:
| D3096-25 | D3096-29 | |
|---|---|---|
| Well Shape | Square | Round |
| Well Bottom | U-bottom | Flat |
| Volume | 1.2mL | 1.2mL |
| Sterile | No | No |
| Case Qty | 50 | 50 |
| Best For | Bacterial culture, mixing, resuspension — square well volume advantage | OD reading, absorbance, optical detection |
D3096-25 vs. D3096-21 — Square U-Bottom 1.2mL vs. Round U-Bottom 1.1mL:
| D3096-25 | D3096-21 | |
|---|---|---|
| Well Shape | Square | Round |
| Volume | 1.2mL | 1.1mL |
| Well Bottom | U-bottom | U-bottom |
| Sterile | No | No |
| Best For | Higher volume + square well format | Standard round well bacterial culture |
| Case Qty | 50 | 50 |
D3096-25 in the Full OptiWell™ 96-Well Non-Sterile Deep Well Family:
| Cat. # | Wells | Bottom | Volume | Sterile | Case Qty |
|---|---|---|---|---|---|
| D3096-09 | 96 Square | V-bottom | 0.5mL | No | 100 |
| D3096-19 | 96 Square | V-bottom | 1.0mL | No | 100 |
| D3096-21 | 96 Round | U-bottom | 1.1mL | No | 50 |
| D3096-23 | 96 Round | V-bottom | 1.1mL | No | 50 |
| D3096-25 | 96 Square | U-bottom | 1.2mL | No | 50 |
| D3096-29 | 96 Round | Flat bottom | 1.2mL | No | 50 |
| D3096-41 | 96 Square | U-bottom Standard | 2.2mL | No | 50 |
| D3096-43 | 96 Square | U-bottom H-style | 2.2mL | No | 50 |
| D3096-45 | 96 Square | V-bottom | 2.3mL | No | 50 |
Automated Platform Compatibility — ANSI/SLAS 1-2004 Standard For automated research platforms and robotic equipment. The D3096-25 conforms to ANSI/SLAS 1-2004 standard 96-well microplate footprint dimensions — ensuring compatibility with all major automated liquid handling platforms including Hamilton, Tecan, Beckman Coulter, PerkinElmer, Agilent, and other standard HTS robotic systems.
4,000 × g Centrifugation Rating Centrifugation up to 4,000 × g — covering all standard plate centrifugation protocols for bacterial cell pelleting, bead collection, and sample concentration at 1.2mL scale.
Virgin Medical-Grade Polypropylene — Minimal Binding and Liquid Retention Minimal binding / liquid retention. Virgin, medical-grade polypropylene. Certified RNase/DNase free and non-pyrogenic.
Black Printed Alpha-Numerics — Instant Well Identification Black printed alpha-numerics. Molded black alpha-numeric well identification (A through H rows, 1 through 12 columns).
Class 100,000 Cleanroom Production — Certified Purity Plates are produced and packaged in a Class 100,000 clean room. Certified non-pyrogenic and RNase/DNase free.
Compatible Replacement — Cross-Reference Guide
The MTC Bio OptiWell™ D3096-25 is a comparable alternative to non-sterile 96-well square U-bottom 1.2mL deep well plates from several major manufacturers:
- Thermo Fisher AB-0661 — Thermo Scientific 96-well square U-bottom deep well plate, 1.2mL, non-sterile
- Greiner Bio-One 780271 — 96-well deep well plate, square U-bottom, 1.2mL, non-sterile
- Eppendorf® 0030501306 — Eppendorf 96-well deepwell plate, square U-bottom, 1.2mL, non-sterile
Competitor brand names and catalog numbers are referenced for cross-referencing purposes only. Volume specifications may vary. Compatibility should be verified for your specific application and protocol.
Key Features
- MTC Bio OptiWell™ — Catalog # D3096-25
- 96-well deep well plate — square U-bottom wells
- 1.2mL per-well capacity — square U-bottom mid-volume format
- Square U-bottom — maximum volume efficiency with uniform mixing and resuspension
- Non-sterile
- Virgin medical-grade polypropylene — minimal binding and liquid retention
- Black printed alpha-numerics — instant well identification
- ANSI/SLAS 1-2004 compliant — compatible with all standard automated liquid handling platforms
- 4,000 × g centrifugation rating
- Compatible with silicone sealing mats and sealing film
- No lid, no skirt
- Autoclavable — can be sterilized before use if required
- Class 100,000 cleanroom production
- Certified RNase-free and DNase-free
- Certified non-pyrogenic
- Sigma-Aldrich catalog # MTCD309625
- Case of 50 plates — 10 bags of 5 plates
Supported Applications Non-sterile bacterial liquid culture at 1.2mL scale with square well volume advantage, cell suspension culture in 96-well deep well format, non-sterile automated liquid handling sample reformatting, compound dilution and preparation at 1.2mL scale, bead-based assay preparation requiring U-bottom mixing and resuspension, centrifugation workflows up to 4,000 × g, general sample storage and archiving, and any 96-well application requiring non-sterile square U-bottom deep well format with 1.2mL per-well capacity and full automated platform compatibility.
Technical Specifications
| Specification | Value |
|---|---|
| SKU / Catalog # | D3096-25 |
| Sigma-Aldrich Catalog # | MTCD309625 |
| Manufacturer | MTC Bio |
| Product Line | OptiWell™ |
| Well Format | 96-well |
| Well Shape | Square |
| Well Bottom | U-bottom (round) |
| Well Volume / Capacity | 1.2mL per well |
| Material | Virgin medical-grade polypropylene |
| Color | Clear |
| Alpha-Numerics | Yes — black printed |
| Lid | No |
| Skirt | No |
| Centrifugation Rating | 4,000 × g |
| Binding | Minimal — low binding and liquid retention |
| Sealing Compatibility | Silicone sealing mats and sealing film |
| Sterility | Non-sterile |
| Autoclavable | Yes |
| Footprint | ANSI/SLAS 1-2004 compliant |
| Production Environment | Class 100,000 cleanroom |
| RNase-Free | Yes — certified |
| DNase-Free | Yes — certified |
| Non-Pyrogenic | Yes — certified |
| Case Quantity | 50 plates (10 bags × 5 plates) |